Cloning and Sequence Analysis of rbcS Gene of Wild Barley (Hordeum brevisubulatum) under Salt Stress

作     者：岳海燕 尹剑锐 闫守庆 冯宇隆 张莲姬 郭建强 李怀亮 丁雪梅 沈景林 YUE Hai-yan;YIN Jian-rui;YAN Shou-qing;FENG Yu-long;ZHANG Lian-ji;GUO Jian-qiang;LI Huai-liang;DING Xue-mei;SHEN Jing-lin

作者机构：吉林大学畜牧兽医学院吉林长春130062 

基　　金：Supported by National Natural Science Foundation of China(30471229 ) National High Technology Research and Development Program "863" Project(2008AA10Z224) Students Innovative Experimental Projects in Jilin University (2009C81147) 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2010年第11卷第8期

页      码：42-44页

摘      要：[Objective] The aim was to study the cloning and sequence analysis of rbcS gene of wild barley under salt stress. [Method] The tender leaf blade of wild barley under salt stress was taken as the experimental material. The primers were designed according to the homology of rbcS gene sequences of wheat and barely in Genbank; then PCR amplification,recovery,ligation,transformation and sequencing of rbcS gene were carried out. [Result] Two rbcS genes including rbcS1 and rbcS2 with the length of 1 252 and 908 bp respectively were cloned from the barely genome. rbcS1 and rbcS2 were both composed by two exons and one intron. The exons length of the two genes was the same of 525 bp,encoding 174 amino acids,and the homology between them was 96%; however,the intron length of rbcS1 and rbcS2 was 448 and 107 bp respectively.

主 题 词：Wild barley Salt stress Ribulose-1 5-Bisphosphate Carboxylase/Oxygenase Small Subunit Sequence analysis 

学科分类：09[农学] 0901[农学-植物生产类] 

D　O　I：10.3969/j.issn.1009-4229-B.2010.08.012

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