Optimization and Testing for PCR System of Rice by Orthogonal Design

作     者：程芳艳 李春光 刘永巍 孟巧霞 张景龙 宋冬明 刘华招 孟昭河 Cheng Fang-yan;LI Chun-guang;LIU Yong-wei;MENG Qiao-xia;ZHANG Jing-long;SONG Dong-ming;LIU Hua-zhao;MENG Zhao-he

作者机构：黑龙江省农垦科学院水稻研究所黑龙江佳木斯154007 

基　　金：Supported by the Key Project from Department of Science and Technology of Heilongjiang Province(GA06B102-3-4) the Key Project from Heilongjiang Provincial Reclamation General Administration(HNKXIV-01-01-02)~~ 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2010年第11卷第2期

页      码：61-64页

摘      要：[Objective] This study was to screen the economic or stable PCR system of rice and detect the generality of the selected system in different molecular markers based on PCR.[Method] With DNA extracted from rice leaves by CTAB method as the template,PCR system was optimized by L16(45)orthogonal design.[Result] Clear bands were amplified from 16 different combinations,but the amplification effects and yields had *** most economic and applicable system was as follows:20 ng DNA template,150 μmol/L dNT...

主 题 词：Rice PCR system optimization Orthogonal design Dominant molecular markers testing 

学科分类：09[农学] 0901[农学-植物生产类] 

D　O　I：10.3969/j.issn.1009-4229-B.2010.02.014

馆 藏 号：203638798...