Establishment of Multiplex PCR for Three Virus of Potato

作     者：乔楠 曹佳 李霞 

作者机构：榆林学院生命科学学院陕西榆林719000 北京真思维生物科技有限公司北京100044 陕西省榆林市农业科学研究所陕西榆林719000 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2011年第12卷第8期

页      码：1209-1212页

摘      要：[Objective]The aim was to establish the multiplex PCR method for three virus of potato：PVA（potato virus A）,TMV（Tobacco mosaic virus）and PVY（potato virus Y）.[Method]According to the PVA,TMV and PVY sequences available in GenBank,pairs of primer were designed for establishing a multiplex PCR method,and constructing recombinant plasmid of target genes by PCR amplified of three viruses as reference standard simple to be used in sensitivity test;PVX（Potato virus X）,PVM（Potato virus M）,PVS（Potato virus S）,PVV（Potato virus V）and CMV（Cucumber mosaic virus）were used to carry out the specificity test and detection of 11 samples which were suspected of virus infected.[Result]The detection limit for PVA,TMV and PVY was 14,14 and 14 copies/ml,*** cross-reactivity was observed with other *** of 11 samples were infected by three viruses.[Conclusion]The multiplex PCR for PVA,TMV,PVY three viruses of potato was established successfully,which had provided basis for the detection technology of potato virus.

主 题 词：Potato Potato virus A Potato virus Y Tobacco mosaic virus Multiplex PCR 

学科分类：09[农学] 0904[农学-动物医学类] 090401[090401] 090402[090402] 

D　O　I：10.3969/j.issn.1009-4229-B.2011.08.035

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