Identification of Genetic Variation in Citrus sinensis from Hunan Based on Start Codon Targeted Polymorphism

作     者：蒋巧巧 龙桂友 李武文 邓子牛 

作者机构：湖南农业大学园艺园林学院湖南长沙410128 国家柑橘改良中心长沙分中心湖南长沙410128 湖南省作物种质创新与资源利用重点实验室湖南长沙410128 湖南永兴县农业局湖南永兴423300 

基　　金：Supported by National Key Technology Research and Development Program(2006BAD01A1702) 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2011年第12卷第11期

页      码：1594-1599页

摘      要：[Objective] The aim was to identify genetic variation in Citrus sinensis （sweet orange） germplasm from Hunan Province according to the Start Codon Targeted （SCoT） Polymorphism. [Method] The reaction system for SCoT amplification from sweet orange was first optimized, and then the SCoT fragments were amplified from 24 sweet orange cultivars collected in Hunan Province and sequenced for genetic variation analysis. [Result] The optimum reaction system for SCoT markers amplification was 2.0 μl containing 80 ng of template DNA, 0.3 mmol/L dNTPs, 0.2 μmol/L primer, 1.6 mmol/L Mg2＋, 1.6 U of Taq DNA polymerase and 10×PCR buffer. By using this reaction system, the PCR products from the sweet orange cultivars produced clear and reproducible bands at 100-2 000 bp through electrophoresis. The SCoT fragments of the 24 sweet orange cultivars were 1 090-1 091 bp, with the homology of 99.84% and nucleotide deletion and substitution. After being sequenced, the SCoT polymorphisms could distinguish 12 sweet orange cultivars. In addition, the BLAST result showed that part of the SCoT fragments coding region shared high homology with ribosomal protein S3 N superfamily. [Conclusion] This study will provide a theoretical basis for breeding sweet orange cultivars.

主 题 词：Citrus sinensis (L.) Osbeck SCoT Uniform design Sequence alignment 

学科分类：09[农学] 0902[农学-自然保护与环境生态类] 090201[090201] 

D　O　I：10.3969/j.issn.1009-4229-B.2011.11.010

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