Cloning of WRKY2 Gene in Tomato

作     者：李娜 于涌鲲 赵福宽 汤世坤 孙清鹏 于同泉 路苹 

作者机构：北京农学院生物技术学院北京102206 农业应用新技术北京市重点实验室北京102206 

基　　金：Supported by Beijing Nature Science Foundation(5102015) 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2011年第12卷第8期

页      码：1125-1128页

摘      要：[Objective]The aim was to explore the function of WRKY transcription factor in tomato.[Method]The primers were designed in this study according to the obtained WRKY fragments,and the total RNA from tomato treated with 100 μmol/L of JA for 6 h was used as the template for RT-PCR.[Result]The 608 bp fragment was obtained from tomato with RT-PCR *** analysis indicated that this sequence contained WRKYGQK conservative domain and the similarity with Capsicum annuum WRKY-c and Nicotiana tabacum NtWRKY-7 were 79% and 74%,respectively.[Conclusion]WRKY gene sequence in tomato was cloned successfully.

主 题 词：Tomato WRKY transcription factor WRKY2 gene Cloning 

学科分类：09[农学] 0902[农学-自然保护与环境生态类] 090202[090202] 

D　O　I：10.3969/j.issn.1009-4229-B.2011.08.012

馆 藏 号：203734140...