Optimization of the Conditions of SRAP Molecular Marker Used in Analysis of Fagopyrum tataricum

作     者：王耀文 夏楠 韩瑞霞 李艳琴 王安虎 蔡光泽 WANG Yao-wen;XIA Nan;HAN Rui-xia;LI Yan-qin;WANG An-hu;CAI Guang-ze

作者机构：山西大学生物技术研究所化学生物学与分子工程教育部重点实验室山西太原030006 西昌学院四川西昌615013 

基　　金：Supported by National Natural Science Foundation of China(30771310) 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2010年第11卷第9期

页      码：32-36,179页

摘      要：[Objective] The aim was to investigate the optimal conditions of SRAP molecular marker used in the analysis on Fagopyrum tataricum（L.）Gaertn.[Method]SRAP-PCR amplification system on Fagopyrum tataricum was optimized by interactive orthogonal design L27（313）in 5 elements（Mg2＋,dNTP,Taq DNA polymerase,template DNA and primer）at 3 *** the non-denaturing and denaturing PAGE detection methods were *** comparative test of DYCZ-24F and DYCZ-20C electrophoresis operating systems was carried out.[Result]The effects of four single-factor（Mg2＋,dNTP,Taq DNA polymerase and primer）and two interactions（Mg2＋×dNTP,Mg2＋×Taq DNA polymerase）on tartary buckwheat SRAP-PCR were *** optimal reaction system was established containing 1.5 mmol/L Mg2＋,0.2 mmol/L dNTP,1.5 u Taq DNA polymerase,40 ng DNA,0.25 μmol/L primer and 2 μl 10×*** samples of tartary buckwheat were amplified using this system,and electrophoresis results showed clear bands,high level of polymorphism and good *** PCR products were tested by denaturing and non-denaturing PAGE,and the results showed that the non-denaturing PAGE,DYCZ-24F operating system was more suitable for SRAP analysis.[Conclusion]This study established a foundation for the construction of SRAP genetic map of tartary buckwheat.

主 题 词：Fagopyrum tataricum SRAP-PCR Interactive orthogonal design Denaturing PAGE Non-Denaturing PAGE 

学科分类：09[农学] 0901[农学-植物生产类] 

D　O　I：10.3969/j.issn.1009-4229-B.2010.09.009

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