Cloning and Sequence Analysis of CmNAC Gene from Cucurbita moschata

作     者：郝芮 赵福宽 孙清鹏 杨爱珍 

作者机构：北京农学院生物技术系北京102206 

基　　金：Supported by Stress Resistant Plants Research Program from Beijing Municipal Commission of Education(5075101019) 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2012年第13卷第2期

页      码：295-298页

摘      要：[Objective] The aim was to clone the CmNAC gene from Cucurbita moschata and analyze the sequence characteristics. [Method] A pair of degenerate primers was designed based on the conserved sequences of NAC gene from Brassica napus, Lycopersicon esculentum and Capsicum annuum. NAC transcription factor gene was amplified by RT-PCR from Cucurbita moschata leaves and cloned into pMD-19T vector; then the recombinant clones were sequenced. Finally, the sequences of nucleic acid and amino acid were analyzed using BLAST and DNAMAN software. [Result] The NAC transcription factor gene cloned from C. moschata included 442 bp encoding 147 amino acids, named CmNAC. The NAC gene fragment contained a conserved region like other plant NAC genes and belonged to the NAC family ATAF1/2 subfamily. [Conclusion] The stress resistance related gene NAC cloned from C. moschata is a foundation for further study on the biological function of the gene and plant genetic engineering.

主 题 词：Cucurbita moschata NAC Gene cloning Sequence analysis 

学科分类：09[农学] 0902[农学-自然保护与环境生态类] 090202[090202] 

D　O　I：10.3969/j.issn.1009-4229-B.2012.02.012

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