Research on the Fermentation Conditions of Bacillus subtillus B-332

作     者：刘雪 叶婧 穆长青 朱昌雄 Xue LIU;Jing YE;Changqing MU;Changxiong ZHU

作者机构：中国农业科学院农业环境与可持续发展研究所北京100081 北京市植物保护站北京100029 

基　　金：Supported by Major Science and Technology Project of National Water Pollution Control and Management "Pollution Control against Agricultural Non-point Source Pollution into River and Ecological Restoration Technology and Engineering Demonstration Project"(2009ZX07103-002) "the Eleventh Five-Year" National Key Technology Support Program "Control Technology and Demonstration of Overall Planning of Urban and Rural Life Rubbish Pollution" (2009BADC2B00-02)~~ 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2013年第14卷第1期

页      码：81-85,106页

摘      要：[Objective] The paper was to ascertain the fermentation conditions and medium formula of Bacillus subtilis B-332. [Method] Using single factor and orthogo- nal experimental designs, the fermentation medium and culture conditions for B. sub- i tills B-332 were optimized through shaking flask culture. [Result] The optimal culture and inoculation time was 18 h, and the optimized medium formula was as follows： soybean powder 0.60 g/L, sucrose 0.25 g/L, ammonium sulfate 0.07 g/L, trisodium citrate 0.03 g/L, dipotassium hydrogen phosphate 0.003 g/L, magnesium sulfate 0.005 g/L, ferrous sulfate 0.000 5%; the fermentation condition was as follows： tem- perature 30 ℃, shaking bed speed 180 r/min, shaking bottle volume 80 ml/500 ml. In the optimal fermentation conditions, the fermented spore quantity was 1.43×10^11 cfu/ml, which was 34.48 times higher than the spore quantity of 4.03×10^9 cfu/ml in initial condition. [Result] The study laid the foundation for factory production of the strain.

主 题 词：Bacillus subtillus Fermentation condition Medium Optimization 

学科分类：09[农学] 0904[农学-动物医学类] 

D　O　I：10.3969/j.issn.1009-4229(e).2013.01.017

馆 藏 号：203827150...