Differentiation of smooth muscle progenitor cells in peripheral blood and its application in tissue engineered blood vessels

作     者：Shang-zhe XIE Ning-tao FANG Shui LIU Ping ZHOU Yi ZHANG Song-mei WANG Hong-yang GAO Luan-feng PAN 

作者机构：Laboratory of Molecular Biology Shanghai Medical College Fudan University Shanghai 200032 China Key Laboratory of Molecular Engineering of Polymers Ministry of Education Department of Macromolecular Science Fudan University Shanghai 200433 China 

基　　金：supported by Shanghai Science Committee Fund for Key Research Project (No. 04JC14012) Fudan University Med-X Fund, China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2008年第9卷第12期

页      码：923-930页

摘      要：Background： A major shortcoming in tissue engineered blood vessels （TEBVs） is the lack of healthy and easily attainable smooth muscle cells （SMCs）. Smooth muscle progenitor cells （SPCs）, especially from peripheral blood, may offer an alternative cell source for tissue engineering involving a less invasive harvesting technique. Methods： SPCs were isolated from 5-ml fresh rat peripheral blood by density-gradient centrifugation and cultured for 3 weeks in endothelial growth medium-2-MV （EGM-2-MV） medium containing platelet-derived growth factoroBB （PDGF BB）. Before seeded on the synthesized scaffold, SPC-derived smooth muscle outgrowth cell （SOC） phenotypes were assessed by immuno-fluorescent staining, Western blot analysis, and reverse transcription polymerase chain reaction （RT-PCR）. The cells were seeded onto the silk fibroin-modified poly（3-hydroxybutyrate-co-3-hydroxyhexanoate） （SF-PHBHHx） scaflblds by 6× 10^4 cells/cm^2 and cultured under the static condition for 3 weeks. The growth and proliferation of the seeded cells on the scaffold were analyzed by 3-（4,5-dimethylthiazol-2-yl）-diphenyltetrazolium bromide （MTT） assay, scanning electron microscope （SEM）, and 4,6-diamidino-2-phenylindole （DAPI） staining. Results： SOCs displayed specific ＂hill and valley＂ morphology, expressed the specific markers of the SMC lineage： smooth muscle （SM） a-actin, calponin and smooth muscle myosin heavy chain （SM MHC） at protein and messenger ribonucleic acid （mRNA） levels. RT-PCR results demonstrate that SOCs also expressed smooth muscle protein 22a （SM22a, a contractile protein, and extracellular matrix components elastin and matrix Gla protein （MGP）, as well as vascular endothelial growth factor （VEGF）. After seeded on the SF-PHBHHx scaffold, the cells showed excellent metabolic activity and proliferation. Conclusion： SPCs isolated from peripheral blood can be differentiated the SMCs in vitro and have an impressive growth potential in the biodegradable synthesized scaffold. Thus,

主 题 词：Smooth muscle progenitor cells (SPCs) Tissue-engineered blood vessels (TEBVs) Silk fibroin (SF) Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) 

学科分类：0710[理学-生物科学类] 071010[071010] 07[理学] 

核心收录：

D　O　I：10.1631/jzus.B0820257

馆 藏 号：203856009...