松毛翠的离体快繁体系建立及种质试管保存

作     者：顾地周 罗微 曹逊 姜云天 朱俊义 Gu Dizhou;Luo Wei;Cao Xun;Jiang Yuntian;Zhu Junyi

作者机构：通化师范学院生物系通化134002 东北师范大学生命科学学院长春130024 

基　　金：吉林省科技厅自然科学基金资助项目(200705C05) 通化师范学院自然科学基金资助项目(XS070079) 

出 版 物：《林业科学》 (Scientia Silvae Sinicae)

年 卷 期：2009年第45卷第7期

页      码：140-144页

摘      要：The tender buds of Phyllodoce caerulea were used as explants for this *** most suitable culture media were screened for shoots regeneration directly from bases of the tender buds,rooting and germplasm preservation in vitro with a uniform *** results showed that DR+TDZ4.00 mg·L-1was the most suitable for shoots regeneration,and the rate of regeneration was more than 98.5%.MS（modified）+IBA0.05 mg·L-1+NAA0.01 mg·L-1+KT0.10 mg·L-1was the most suitable for rooting,and the rate of rooting was more than 97.8%.N-68+B92.30 mg·L-1+ phloridzin 1.50 mg·L-1was the most suitable for preservation in vitro for 42 *** each with one node were cut from the regenerated shoots and cultured for propagation,and a 35-fold proliferation rate was achieved within 40 *** method of "deferring growth with dwarfing" was utilized for germplasm preservation in vitro at normal *** vitro culture and germplasm preservation in vitro system of Phyllodoce caerulea had been successfully established.

主 题 词：松毛翠 种质试管保存 均匀设计 根皮苷 

学科分类：0710[理学-生物科学类] 090701[090701] 0907[农学-草药学] 07[理学] 09[农学] 071007[071007] 0901[农学-植物生产类] 090102[090102] 

核心收录：

D　O　I：10.3321/j.issn:1001-7488.2009.07.024

馆 藏 号：203856394...