Designer cell-self-implemented labeling of microvesicles in situ with the intracellular-synthesized quantum dots

作     者：Ling-Hong Xiong Jia-Wei Tu Ya-Nan Zhang Ling-Ling Yang Ran Cui Zhi-Ling Zhang Dai-Wen Pang Ling-Hong Xiong;Jia-Wei Tu;Ya-Nan Zhang;Ling-Ling Yang;Ran Cui;Zhi-Ling Zhang;Dai-Wen Pang

作者机构：College of Chemistry and Molecular SciencesState Key Laboratory of VirologyInstitute for Advanced Studiesand Wuhan Institute of BiotechnologyWuhan UniversityWuhan 430072China State Key Laboratory of Medicinal Chemical BiologyTianjin Key Laboratoryof Biosensing and Molecular RecognitionResearch Center for Analytical Sciencesand College of ChemistryNankai UniversityTianjin 300071China 

基　　金：the National Natural Science Foundation of China(21535005 91859123 21705111) 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2020年第63卷第4期

页      码：448-453页

摘      要：Cell-derived microvesicles(MVs) are secreted from almost all kinds of mammalian cells into the extracellular space, and play crucial roles in intercellular communication and transporting biomolecules between cells. However, there is a great challenge for visualizing and monitoring of MVs’ bio-behaviors due to the limitations of existing labeling methods. Herein, we report the first paradigm of designer cell-self-implemented labeling of MVs secreted from living mammalian MCF-7 cells in situ using the intracellular-synthesized fluorescent quantum dots(QDs) during the formation of MVs. By elaborately coupling intracellular biochemical reactions and metabolism pathways, the MCF-7 cells can be illuminated brightly by intracellular-biosynthesized fluorescent CdSe QDs. Simultaneously, intracellular-synthesized QDs can be in situ encapsulated by the secreted MVs budding from the plasma membrane of the fluorescing cells to label the MVs with an efficiency of up to 89.9%. The whole labeling process skillfully combines designer precise cell-tuned intricate synthesis of CdSe QDs with mild in-situ labeling via cell-selfimplementation just after feeding the cell with suitable chemicals, which is structure-or function-nondestructive and much more straightforward and milder than those by chemical conjugation or indirect encapsulation with conventional fluorogenic labels.

主 题 词：in situ labeling live cell synthesis cell-derived microvesicles quantum dot cell-self-implementation 

学科分类：0710[理学-生物科学类] 07[理学] 071009[071009] 09[农学] 0901[农学-植物生产类] 090102[090102] 

核心收录：

D　O　I：10.1007/s11426-019-9697-2

馆 藏 号：203884732...