Design, synthesis and systematic evaluation of all possible cyclic dinucleotides (CDNs) that activate human stimulator of interferon genes (STING) variants

作     者：Zheng-Hua Wang Can-Can Zhao Qiang-Zhe Zhang Chuan-Lin Wang Hang Zhang De-Jun Ma Da-Wei Wang Xin Wen Lu-Yuan Li Zhen Xi Zheng-Hua Wang;Can-Can Zhao;Qiang-Zhe Zhang;Chuan-Lin Wang;Hang Zhang;De-Jun Ma;Da-Wei Wang;Xin Wen;Lu-Yuan Li;Zhen Xi

作者机构：State Key Laboratory of Elemento-organic ChemistryDepartment of Chemical BiologyCollege of ChemistryNankai UniversityTianjin 300071China State Key Laboratory of Medicinal Chemical Biology and College of PharmacyTianjin Key Laboratory of Molecular Drug ResearchNankai UniversityTianjin 300071China Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)Tianjin 300071China National Pesticide Engineering Research Center(Tianjin)Tianjin 300071China 

基　　金：the National Key Research and Development Program of China(2017YFD0200500) the National Natural Science Foundation of China(21740002,21837001) 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2020年第63卷第4期

页      码：534-545页

摘      要：Cyclic dinucleotides(CDNs) are known to activate stimulator of interferon genes(STING) and induce type I interferon responses, therefor possess great potentials to be of immunotherapeutic value for cancers and infectious diseases. However, the existence of different single nucleotide polymorphism(SNP) of human STING(hSTING) gene poses an obstacle to achieve broad-spectrum activation by CDNs. We reported here the design and synthesis of a total of 36 CDNs, representing all structural variations, that contain four bases(A, G, C, U) and two linkage directions(2′-5′-linked and 3′-5′-linked phosphodiester).Through systematic evaluation of IFN-β induction with a dual-luciferase reporter assay, we discovered that wild type hSTING and two isoforms(HAQ and AQ) showed strong response while hSTING-R232 H and R293 Q exhibited the relatively weak response to CDNs stimulation. For the first time, we found that the c[G(2′,5′)U(2′,5′)] showed excellent activity against all five hSTING variants even equivalent to the endogenous ligand c[G(2′,5′)A(3′,5′)]. Furthermore, we have also demonstrated that 3′-3′CDNs with two 3′-5′ phosphodiesters showed higher serum and hydrolase stability than 2′-2′ CDNs with two 2′-5′ phosphodiesters and 2′-3′ CDNs with one 2′-5′ and one 3′-5′ phosphodiester. It is very interesting to note that 2′-2′ CDNs has been found for the first time to show strong activity. These findings will stimulate our exploration for the new functional role of CDNs, and provide guidelines to design CDNs based hSTING targeted drugs.

主 题 词：cyclic dinucleotides(CDNs) stimulator of interferon genes(STING) pyrimidine CDNs interferonβ ecto-nucleotide pyrophosphatase/phosphodiesterase 1(ENPP1) 

学科分类：1007[医学-药学类] 10[医学] 

核心收录：

D　O　I：10.1007/s11426-019-9662-5

馆 藏 号：203884920...