Redesigned Duplex RT-qPCR for the Detection of GI and GII Human Noroviruses

作     者：Danlei Liu Zilei Zhang Qingping Wu Peng Tian Haoran Geng Ting Xu Dapeng Wang Danlei Liu;Zilei Zhang;Qingping Wu;Peng Tian;Haoran Geng;Ting Xu;Dapeng Wang

作者机构：Department of Food Science and TechnologySchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Applied Microbiology Southern China&Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application&Guangdong Open Laboratory of Applied Microbiology&Guangdong Institute of MicrobiologyGuangzhou 510070China Produce Safety and Microbiology Research UnitWestern Regional Research CenterAgricultural Research ServiceUnited States Department of AgricultureAlbanyCA 94706USA 

基　　金：supported by the Ministry of Science and Technology of China(2017YFC1601200) the National Natural Science Foundation of China(31772078) the Agri-X Interdisciplinary Fund of Shanghai Jiao Tong University(2017) 

出 版 物：《Engineering》 (工程（英文）)

年 卷 期：2020年第6卷第4期

页      码：442-448页

摘      要：Human noroviruses(HuNoVs)are major foodborne pathogens that cause nonbacterial acute gastroenteritis *** the tissue-culture system for HuNoVs is not mature enough for routine detection of the virus,detection is mainly dependent on molecular approaches such as reverse transcription polymerase chain reaction(RT-PCR)and reverse transcription quantitative real-time polymerase chain reaction(RTqPCR).The widely used primers and probes for RT-qPCR were established in the early *** HuNoVs are highly variant viruses,viral genome mutations result in previously designed primers and/or probes that were perfectly matched working less efficiently over *** this study,a new duplex RT-qPCR(ND-RT-qPCR)was designed for the detection of genogroup Ⅰ(GⅠ)and genogroup Ⅱ(GⅡ)HuNoVs based on an analysis of viral sequences added in the database after *** long transcribed viral RNAs,the results demonstrate that the sensitivity of ND-RT-qPCR is as low as one genomic copy for both GⅠ and GⅡ *** performance of ND-RT-qPCR was further evaluated by a comparison with the commonly used Kageyama primer/probe sets for RT-qPCR(Kageyama RT-qPCR)for 23 HuNoV-positive clinical *** five GⅠ samples were registered as positive by ND-RT-qPCR,whereas only two samples were registered as positive by Kageyama *** 18 GⅡ samples were registered as positive by ND-RT-qPCR,while 17 samples were registered as positive by Kageyama *** sensitivity reflected by the quantification cycle(Cq)value was lower in ND-RT-qPCR than in Kageyama *** data suggest that ND-RT-qPCR could be a good fit for the detection of current strains of HuNoVs.

主 题 词：Human noroviruses RT-qPCR Redesign Primer Probe Detection 

学科分类：0808[工学-自动化类] 0810[工学-土木类] 100208[100208] 1007[医学-药学类] 0830[工学-生物工程类] 100705[100705] 1002[医学-临床医学类] 1001[医学-基础医学] 0817[工学-轻工类] 0807[工学-电子信息类] 0805[工学-能源动力学] 100103[100103] 0703[理学-化学类] 0812[工学-测绘类] 10[医学] 

核心收录：

D　O　I：10.1016/j.eng.2019.08.018

馆 藏 号：203887799...