Development of genic SSR markers from transcriptome sequencing of pear buds

作     者：Xiao-yan YUE Guo-qin LIU Yu ZONG Yuan-wen TENG Dan-ying CAI 

作者机构：State Agricultural Ministry Key Laboratory of Horticultural Plant GrowthDevelopment&Quality ImprovementDepartment of HorticultureZhejiang University College of AgricultureGuizhou University 

基　　金：supported by the National Natural Science Foundation of China(No.31201592) the Modern Agro-industry Technology Research System(No.nycytx-29-14) the Doctoral Program of Higher Education(No.20110101110091),China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2014年第15卷第4期

页      码：303-312页

摘      要：A total of 8375 genic simple sequence repeat（SSR） loci were discovered from a unigene set assembled from 116282 transcriptomic unigenes in this *** repeat motifs were the most common with a frequency of 65.11%,followed by trinucleotide（32.81%）.A total of 4100 primer pairs were designed from the SSR *** these,343 primer pairs（repeat length≥15 bp） were synthesized with an M13 tail and tested for stable amplification and polymorphism in four Pyrus *** the preliminary test,104 polymorphic genic SSR markers were developed; dinucleotide and trinucleotide repeats represented 97.11%（101） of ***-eight polymorphic genic SSR markers were selected randomly to further validate genetic diversity among 28 Pyrus *** markers displayed a high level of *** number of alleles at these SSR loci ranged from 2 to 17,with a mean of 9.43 alleles per locus,and the polymorphism information content（PIC） values ranged from 0.26 to *** UPGMA（unweighted pair-group method with arithmetic average） cluster analysis grouped the 28 Pyrus accessions into two groups： Oriental pears and Occidental pears,which are congruent to the traditional taxonomy,demonstrating their effectiveness in analyzing Pyrus phylogenetic relationships,enriching rare Pyrus EST-SSR resources,and confirming the potential value of a pear transcriptome database for the development of new SSR markers.

主 题 词：Genic marker Simple sequence repeat Transcriptome Genetic diversity Pyrus 

学科分类：0710[理学-生物科学类] 1007[医学-药学类] 1004[医学-公共卫生预防医学类] 1001[医学-基础医学] 0905[农学-林学类] 0906[农学-水产类] 09[农学] 0902[农学-自然保护与环境生态类] 090201[090201] 

核心收录：

D　O　I：10.1631/jzus.B1300240

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