A hepatocellular carcinoma–bone metastasis-on-a-chip model for studying thymoquinone-loaded anticancer nanoparticles

作     者：Fatemeh Sharifi Ozlem Yesil-Celiktas Aslihan Kazan Sushila Maharjan Saghi Saghazadeh Keikhosrow Firoozbakhsh Bahar Firoozabadi Yu Shrike Zhang Fatemeh Sharifi;Ozlem Yesil-Celiktas;Aslihan Kazan;Sushila Maharjan;Saghi Saghazadeh;Keikhosrow Firoozbakhsh;Bahar Firoozabadi;Yu Shrike Zhang

作者机构：Division of Engineering in MedicineDepartment of MedicineBrigham and Women’s HospitalHarvard Medical SchoolCambridgeMAUSA School of Mechanical EngineeringSharif University of TechnologyTehranIran Department of BioengineeringFaculty of EngineeringEge UniversityBernovaIzmirTurkey 

基　　金：This work was supported by the National Institutes of Health(K99CA201603,R00CA201603,R21EB025270,R21EB026175,R01EB028143) the New England Anti-Vivisection Society,and the Brigham Research Institute 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2020年第3卷第3期

页      码：189-202页

摘      要：We report the development of a metastasis-on-a-chip platform to model and track hepatocellular carcinoma(HCC)-bone metastasis and to analyze the inhibitory effect of an herb-based compound,thymoquinone(TQ),in hindering the migration of liver cancer cells into the bone *** bioreactor consisted of two chambers,one accommodating encapsulated HepG2 cells and one bone-mimetic niche containing hydroxyapatite(HAp).Above these chambers,amicroporous membrane was placed to resemble the vascular barrier,where medium was circulated over the *** was observed that the liver cancer cells proliferated inside the tumor microtissue and disseminated from the HCC chamber to the circulatory flow and eventually entered the bone *** number of metastatic HepG2 cells to the bone compartment was remarkably higher in the presence of HAp in the *** was then used as ametastasis-controlling agent in both free form and encapsulated nanoparticles,to analyze its suppressing effect on HCC *** indicated that the nanoparticle-encapsulated TQ provided a longer period of inhibitory *** summary,HCC-bone metastasis-on-a-chip platform was demonstrated to model certain key aspects of the cancer metastasis process,hence corroborating the potential of enabling investigations on metastasis-associated biology as well as improved anti-metastatic drug screening.

主 题 词：Tumor-on-a-chip Organ-on-a-chip Hydroxyapatite Hepatocellular carcinoma Thymoquinone 

学科分类：08[工学] 0710[理学-生物科学类] 0831[工学-公安技术类] 1008[医学-中药学类] 1006[医学-中西医结合类] 0817[工学-轻工类] 0807[工学-电子信息类] 080501[080501] 0805[工学-能源动力学] 080502[080502] 0802[工学-机械学] 0836[0836] 0811[工学-水利类] 100602[100602] 10[医学] 

核心收录：

D　O　I：10.1007/s42242-020-00074-8

馆 藏 号：203955492...