MMEJ-KO:a web tool for designing paired CRISPR guide RNAs for microhomology-mediated end joining fragment deletion

作     者：Xianrong Xie Weizhi Liu Gang Dong Qinlong Zhu Yao-Guang Liu Xianrong Xie;Weizhi Liu;Gang Dong;Qinlong Zhu;Yao-Guang Liu

作者机构：State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of Life SciencesSouth China Agricultural UniversityGuangzhou 510642China Guangdong Laboratory for Lingnan Modern AgricultureGuangzhou 510642China 

基　　金：supported by the Key Research Program of Guangzhou Science Technology and Innovation Commission(201904020030) the Major Program of Guangdong Basic and Applied Basic Research(2019B030302006) 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2021年第64卷第6期

页      码：1021-1024页

摘      要：Dear Editor,Clustered regularly interspaced short palindromic repeats associated protein(CRISPR/Cas)systems are convenient and versatile tools for genome *** by a guide RNA(gRNA),the Cas nuclease produces double-strand break(DSB)at the target site,generally resulting in small base insertion/deletion mutations by the non-homologous end joining(NHEJ)DNA repair.

主 题 词：CRISPR/Cas joining versatile 

学科分类：0710[理学-生物科学类] 07[理学] 08[工学] 09[农学] 071007[071007] 0901[农学-植物生产类] 0836[0836] 090102[090102] 

核心收录：

D　O　I：10.1007/s11427-020-1797-3

馆 藏 号：203978354...