Structure-based design,synthesis of novel probes for cytochrome P450 OleT

作     者：Dumei Ma Libo Zhang Yingwu Yin Qian Wang Dumei Ma;Libo Zhang;Yingwu Yin;Qian Wang

作者机构：Department of Chemical and Biochemical EngineeringCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China Department of Chemistry and BiochemistryUniversity of South CarolinaColumbiaSC 29208United States 

基　　金：the support from the State Scholarship Fund of the China Scholarship Council(No.201806310084) 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2021年第32卷第4期

页      码：1466-1469页

摘      要：Cytochrome P450 OleT_(SA),a new cytochrome P450 enzyme from Staphylococcus aureus,catalyzes the oxidative decarboxylation and hydroxylation of fatty acids to generate terminal alkenes and fatty *** mechanism of this bifurcative chemistry remains largely ***,a class of derivatized fatty acids were synthesized as probes to investigate the effects of substrate structure on the product type of P450 OleT_(SA).The results demonstrate that the fine-tuned structure of substrates,even in a remote distance from the carboxyl group,significantly regulates OleT catalyzed decarboxylation/hydroxylation *** docking analysis indicated the potential interactions between the carboxylate groups of different probes and the enzyme active center which was attributed to the bifurcative chemistry.

主 题 词：Substrate engineering Cytochrome P450 OleT Decarboxylation Hydroxylation Fatty acid derivatives 

学科分类：0710[理学-生物科学类] 071010[071010] 081704[081704] 07[理学] 08[工学] 0817[工学-轻工类] 

核心收录：

D　O　I：10.1016/j.cclet.2020.09.042

馆 藏 号：203995066...