Anas platyrhynchos BLVRA Gene:Molecular Cloning and Correlation between Green Shell Character and mRNA Expression in the Shell Gland in the Duck

作     者：ZHANG Bao-le LI Guo-qin ZHANG He SHEN Jun-da LI Jin-jun TAO Zheng-rong CHEN Yi-chu WANG De-qian CHEN Wen-biao SHI Fang-xiong LU Li-zhi 

作者机构：Institute of Animal Husbandry and Veterinary Medicine Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 China Jinyun Husbandry and Veterinary Bureau Jinyun 321403 China Zhejiang Wulian Agriculture And Animal Co. Ltd. Jinyun 321403 China 

出 版 物：《畜牧兽医学报》 (ACTA VETERINARIA ET ZOOTECHNICA SINICA)

年 卷 期：2011年第42卷第B12期

页      码：13-19页

摘      要：This study has examined the structure and function of the biliverdin reductase A (BLVRA) gene and its correlation with the green eggshell character in Jinyun ducks. The previously unknown sequences of 3 and 5 ends of duck BLVRA mRNA were cloned, using RT-PCR and 5 RACE methods with four pairs of primers designed from the known coding sequence (CDS) of the duck BLVRA gene. Gene expression levels of BLVRA in the shell gland were quantified by real-time PCR, and eggshell colors were determined objectively by the reflection coefficient. The cDNA sequence of BLVRA was comprised of 1071 nucleotides, encoding a protein of 303 amino acids with an isoelectric point of 7.15 and molecular weight of 34.3 kDa. The 5 and 3 ends of the duck BLVRA gene sequence published in the GenBank were extended by 238 and 199 bp, respectively. The deduced amino acid sequence of duck BLVRA had 95.3% identity with Gallus gallus, 95.0% with Taeniopygia guttata, 70.0% with Xenopus tropicalis,61.1% with Bos taurus, 60.5% with Homo sapiens and 59.5% with Mus musculus, which indicated that the BLVRA gene was evolutionarily conserved. The relative gene expression level of BLVRA in shell glands of Jinyun ducks with white eggshells was 3 times higher than that in ducks with dark green eggshells (P<0.01). There was a significant positive correlation between reflection coefficient of eggshells and the relative expression of the BLVRA gene (r=0.719, P<0.05). Together, these findings suggest that the BLVRA gene could be a candidate gene for the molecule breeding of ducks with green eggshells.

主 题 词：基因设计 绿头鸭 mRNA表达 克隆 VRA 基因表达水平 GenBank 氨基酸序列 

学科分类：0710[理学-生物科学类] 071010[071010] 081704[081704] 07[理学] 08[工学] 0817[工学-轻工类] 0905[农学-林学类] 09[农学] 

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