摘要：云南省大力推广应用“以油促游、以游带油”的农旅结合发展模式、充分挖掘油菜“菜薹食用、农旅结合、蜂产业、青贮饲料”等多重功能,形成了特色鲜明的云南高原油菜产业。综述了云南油菜产业构建多级联动支撑体系、产学研合作促进科技成果应用、解决了轻简高效生产中的一批关键技术难题、培养了一支研发队伍和一批基层技术人才、打造罗平县“花用+蜜用+油用”农旅结合和腾冲市“花用+油用+菜用+蜜用”综合利用等示范典型,进而形成一批区域产业发展集群的做法和经验,分析了乡村振兴产业发展工作中普遍存在的科技投入、标准化生产经营、全链服务等问题和不足,提出了重视顶层设计,从品种技术上强化科技支撑,集聚社会资源推进油菜产业化经营,促进一二三产业融合,挖掘油菜多功能综合利用价值融入乡村振兴战略等对策与建议,以期为油菜多功能开发利用的深入研究和进一步发展提供参考,有效促进油菜产业的发展和乡村振兴建设。

摘要：以拟南芥(Arabidopsis thaliana)AtFAD2为参考序列,通过BLAST进行同源序列比对,获得FAD2基因在甘蓝(Brassica oleracea)、白菜(Brassica rapa)和甘蓝型油菜(Brassica napus)基因组中的同源拷贝。运用生物信息学方法分析其氨基酸序列的组成、蛋白质基本理化性质、亚细胞定位、亲水性和疏水性、蛋白质二三级结构、保守结构域,并对FAD2基因编辑靶点进行预测及sgRNA设计。在白菜、甘蓝与甘蓝型油菜获得2条FAD2基因同源蛋白质序列;亚细胞定位显示这些AtFAD2同源蛋白质均位于内质网,肽链表现为疏水性、比较稳定;蛋白质序列二三级结构预测表明,主要以α-螺旋和无规则卷曲为组成成分;蛋白质序列保守结构域分析显示,FAD2具有高度的序列保守性,属于Membrane-FADS-like超家族,且含PLN02505保守结构域;系统进化树构建分析显示,甘蓝型油菜和白菜、甘蓝以及芥菜型油菜具有紧密的亲缘关系;CRISPR-Cas9基因编辑靶点分析发现,甘蓝型油菜FAD2基因中存在10段高度保守性序列可作为候选靶点,并设计出相应的sgRNA。

摘要：[Objective] This study aimed to develop ACGM markers for the clustering analysis of large grained Brassica napus materials. [Method] A total of 44 pairs of ACGM primers were designed according to 18 genes related to Arabidopsis grain development and their homologous rape EST sequences. After electrophoresis, 18 pairs of ACGM primers were selected for the clustering analysis of 16 larger grained samples and four fine grained samples of rapeseed. [Result] PCR result showed that 2-6 specific bands were respectively amplified by each pair of primes, and all the bands were polymorphic and repeatable, suggesting that the optimized ACGM markers were useful for clustering analysis of B. napus species. Clustering analysis revealed that the 20 rapeseed samples were divided into three clusters A, B, and C at similarity coefficient 0.6. Then, the clusters A and B were further divided into five sub clusters A1, A2, A3, B1 and B2 at similarity coefficient 0.67. [Conclusion] This study will provide theoretical and practical values for rape breeding.