摘要：本文基于电脑鼠机器人行进过程中的速度控制,将一体式红外线发射接收器探测到的电脑鼠与前方障碍物的距离,以及电机的即时速度作为输入,设计一个模糊控制器,根据输入变量的大小来调整模糊控制器的量化因子,从而自动调整模糊控制策略,输出加速度来控制机器人的行进速度。根据实验,使用模糊自适应控制的电脑鼠机器人性能得到了显著的提高,具有较强的鲁棒性。

摘要：目的:从LPS刺激过的小鼠胸腺细胞中克隆IL-1β基因,通过原核表达,获得具有生物活性的可溶性鼠源IL-1β蛋白,为深入研究和利用IL-1β基因奠定基础。方法:提取LPS刺激的小鼠胸腺细胞总RNA,反转录成cDNA,以此为模板,根据GenBank报道的mIL-1β序列设计引物,进行巢式PCR,得到成熟mIL-1β的编码序列基因,并插入到原核表达载体pHisSUMO ex-press中SUMO标签的下游,构建重组表达载体pHisSUMO express-mIL-1β。将该载体转化大肠杆菌Rosetta(DE3),IPTG诱导表达mIL-1β/SUMO融合蛋白,Ni-NTA Agarose纯化后,表达产物经SDS-PAGE和Western blot进行鉴定,用SUMO protease-1切去SUMO标签,经纯化并切去融合标签后获得成熟mIL-1β蛋白。用MTT方法检测目的蛋白对L929细胞的生物学活性。结果:DNA测序证明所克隆基因序列与GenBank报道的完全一致,SDS-PAGE分析表明融合蛋白的相对分子质量为37kD,切割后的成熟蛋白为17kD,与理论值相符。且蛋白表达量高,主要以可溶形式存在。Western blot证实该蛋白为mIL-1β。成熟蛋白纯化产物纯度超过95%以上,通过MTT的方法检测证明其具有使L929细胞增殖的作用,从而说明其具有生物学活性。结论:利用大肠杆菌表达系统可高效可溶性表达高纯度的具有生物活性的mIL-1β蛋白。

摘要：以O^2-2,4-二硝基苯基偶氮鎓二醇盐(PABA/NO)为先导化合物,选择适当的仲胺作为偶氮鎓二醇盐中相应的胺片段,并用碳氮键取代苯环5位的碳氧酯键,设计合成了化合物2a,2b和4a^4j,以期获得活性更强且稳定性好的抗肿瘤药物.目标化合物经~1H NMR,^(13)C NMR及HRMS进行了结构确证.生物活性测试结果表明,目标化合物可不同程度地抑制结肠癌HCT-116细胞的增殖,其中化合物4h的活性最强(IC50=7.945±0.421μmol/L),优于PABA/NO(IC50=12.134±0.675μmol/L).NO释放实验结果表明,此类化合物的NO释放量与细胞毒性呈正相关.化合物4h在HCT-116细胞中释放NO的量最多,约是正常细胞的2倍.此外,化合物4h在大鼠血浆中的体外稳定性显著优于PABA/NO,值得进一步研究.

摘要：Cu-based catalysts are the most promising candidates for electrochemical CO_(2)reduction(CO_(2)RR)to multi-carbon(C_(2))*** the C-C coupling process,the rate-determining step for C_(2)product generation,is an important strategy to improve the production and selectivity of the C_(2)*** this study,we determined that the local electric field can promote the C-C coupling reaction and enhance CO_(2)electroreduction to C_(2)***,finite-element simulations indicated that the high curvature of the Cu nanoneedles results in a large local electric field on their *** functional theory(DFT)calculations proved that a large electric field can promote C-C *** by this prediction,we prepared a series of Cu catalysts with different *** Cu nanoneedles(NNs)exhibited the largest number of curvatures,followed by the Cu nanorods(NRs),and Cu nanoparticles(NPs).The Cu NNs contained the highest concentration of adsorbed K+,which resulted in the highest local electric field on the *** adsorption sensor tests indicated that the Cu NNs exhibited the strongest CO adsorption ability,and in-situ Fourier-transform infrared spectroscopy(FTIR)showed the strongest*COCO and*CO signals for the Cu *** experimental results demonstrate that high-curvature nanoneedles can induce a large local electric field,thus promoting C-C *** a result,the Cu NNs show a maximum FEC_(2)of 44%for CO_(2)RR at a low potential(-0.6 V ***),which is approximately 2.2 times that of the Cu *** work provides an effective strategy for enhancing the production of multi-carbon products during CO_(2)RR.

摘要：通过对清风公园规划设计的要点分析,以期探讨公园设计与建设的要义与目的,由此引申出公园最终是功能、艺术、生态的综合体,并为今后更好地从人文主义出发,创作宜人的当代优秀公园绿地作一些探索。

摘要：首先总结提炼出核心专利的主要特征,即具有原创性、不可替代性、原理设计科学优化及蕴含着重大战略意义;其次,还厘清了核心专利与相关概念的关系,如核心技术、重要专利、一般专利、外围专利等;最后,通过文献调研,对比分析和分类统计等方法,归纳总结出目前常用的识别核心专利的方法可分为专家智慧法、单一指标法、指标组合法和指标体系法,并发现指标组合和指标体系法越来越被重视。而具体的识别指标来自专利的技术属性、法律属性、商业属性和受重视程度等四个角度,通过比较分析各指标的查全性、准确性及可操作性,研究得出最主要的识别指标有专利被引频次、专利同族家族数量、申请人和发明人数量等。

摘要：Electrochemical CO2 reduction is a promising strategy for the utilization of CO2 and intermittent excess *** is the only single metal catalyst that can electrochemically convert CO2 into multicarbon ***,Cu exhibits an unfavorable activity and selectivity for the generation of C2 products because of the insufficient amount of CO*provided for the C‐C *** on the strong CO2 adsorption and ultrafast reaction kinetics of CO*formation on Pd,an intimate CuPd(100)interface was designed to lower the intermediate reaction barriers and improve the efficiency of C2 product *** functional theory(DFT)calculations showed that the CuPd(100)interface enhanced the CO2 adsorption and decreased the CO2*hydrogenation energy barrier,which was beneficial for the C‐C *** potential‐determining step(PDS)barrier of CO2 to C2 products on the CuPd(100)interface was 0.61 eV,which was lower than that on Cu(100)(0.72 eV).Encouraged by the DFT calculation results,the CuPd(100)interface catalyst was prepared by a facile chemical solution method and characterized by transmission electron ***2 temperature‐programmed desorption and gas sensor experiments further confirmed the enhancement of the CO2 adsorption and CO2*hydrogenation ability of the CuPd(100)interface ***,the obtained CuPd(100)interface catalyst exhibited a C2 Faradaic efficiency of 50.3%±1.2%at‒1.4 VRHE in 0.1 M KHCO3,which was 2.1 times higher than that of the Cu catalyst(23.6%±1.5%).This study provides the basis for the rational design of Cu‐based electrocatalysts for the generation of multicarbon products by fine‐tuning the intermediate reaction barriers.

摘要：黄瓜细菌性白枯病是黄瓜生产上的重要细菌病害,通过病菌16S rDNA序列测定、相关序列比对、系统进化树构建及特异性引物设计研究,明确该病菌系统进化关系,建立PCR检测方法。经NCBI-BLASTn结果表明:黄瓜细菌性白枯病菌CU-PV 07菌株的16S rDNA基因序列与GenBank中的同种相关序列存在较少碱基的差异,同源性达到了99%。设计的引物P1/P2具有很强的特异性,PCR扩增后只有该病菌呈阳性。构建的PCR检测体系检测准确、灵敏度高,可用于此病害快速诊断。

摘要：采用“闷罐车”技术,组建了梨黑星病分生孢子侵染动态预测模型(PSCIM)。PSCIM能以3min为一个步长计算梨黑星病菌分生孢子的侵染量,可以预测梨黑星病菌分生孢子在一个降雨过程中的相对侵染量。PSCIM采用了面向对象的程序设计方法,程序包括主窗体、驱动变量输入窗体和计算单元3个模块,能以图形和数据列表两种方式显示结果。PSCIM在Delphi 6.0实现编译,程序的结构清新自然,能较好地再现梨黑星病分生孢子的侵染过程。