摘要：乙肝病毒X蛋白(Hepatitis B virus X protein,HBx)是乙肝病毒诱导肝癌进展的关键因子,其能够影响肝癌细胞周期和促进其迁移。已知趋化因子受体3(chemokine CXC motif receptor 3,CXCR3)在乙型肝炎病毒肝癌中发挥促进作用,目前尚不清楚CXCR3在HBx诱导的肝癌细胞中的作用。因此,本研究探讨下调CXCR3对HBx诱导的肝癌细胞周期进展和迁移作用及机制。肝癌细胞Huh7分成对照组,pcDNA组(转染阴性对照载体),pcDNAHBx组(转染HBx过表达载体),pcDNA-HBx+si-NC组(共转染HBx过表达载体、siRNA对照的),pcDNA-HBx+si-CXCR3组(共转染HBx过表达载体、CXCR3 siRNA的),pcDNA-HBx+si-CXCR3+Jagged1组(共转染HBx过表达载体、CXCR3 siRNA,Notch1信号激活剂Jagged1处理),测定细胞中HBx、CXCR3、神经性钙黏附素(Neural cadherin,N-cadherin)、基质金属蛋白酶-9(Matrix metalloprotease 9,MMP-9)、细胞周期蛋白D1(cyclin D1)、增殖细胞核抗原(PCNA)、基质金属蛋白酶-2(Matrix metalloprotease 2,MMP-2)、上皮性钙黏附素(Epithelical cadherin,Ecadherin)、波形蛋白(Vimentin)、Notch神经同源蛋白1前体(Neurogenic locus notch homolog protein 1 precusor,Notch1)、Hes1蛋白表达,检测细胞增殖、周期和迁移、侵袭情况。结果表明,与Control、pcDNA组比较,pcDNAHBx组肝癌细胞中HBx、CXCR3、Notch1、Hes1、Cyclin D1、PCNA、MMP-9、MMP-2、vimentin、N-cadherin蛋白表达量升高,细胞增殖活性、迁移数目、侵袭数目升高,细胞G0/G1期比例、E-cadherin蛋白表达量降低(P<0.05);与pcDNA-HBx+si-NC组比较,pcDNA-HBx+si-CXCR3组肝癌细胞中CXCR3、Notch1、Hes1、Cyclin D1、PCNA、MMP-9、MMP-2、vimentin、N-cadherin蛋白表达量降低,细胞增殖活性、迁移数目、侵袭数目降低,细胞G0/G1期比例、E-cadherin蛋白表达量升高(P<0.05);与pcDNA-HBx+si-CXCR3组比较,pcDNA-HBx+si-CXCR3+Jagged1组肝癌细胞Notch1、Hes1、Cyclin D1、PCNA、MMP-9、MMP-2、vimentin、N-cadherin蛋白表达量升高,细胞增殖活性、迁移数目、侵袭数目升高,细胞G0/G1期比例、E-cadherin蛋白表达量降低(P<0.05)。结果表明,下调CXCR3通过抑制Notch1信号减弱HBx促进肝癌细胞周期进展和迁移作用。本研究证明了下调CXCR3对HBx促进肝癌细胞周期进展、迁移、侵袭和EMT的作用,并首次发现了其作用机制与Notch1信号有关。

摘要：目的:本研究旨在综述网络认知行为疗法(ICBT)在青少年焦虑和抑郁症患者中的应用及效果,以期为ICBT在国内的推广提供理论依据。方法:通过检索相关文献,分析ICBT在青少年焦虑和抑郁症中的应用现状、疗效和优势,重点关注自助式ICBT、治疗师有限支持的ICBT和混合式ICBT三种干预模式的特点与挑战。结果:文献分析表明,ICBT能够有效减少青少年的焦虑和抑郁症状,尤其在提高心理健康服务的可及性、降低社会污名化以及提供个性化支持方面展现了优势。然而,依从性差、长期效果不明确以及文化适应性问题仍需进一步研究和优化。结论:ICBT作为青少年焦虑抑郁症的有效干预手段,具有广泛的应用潜力。未来需要通过优化ICBT平台设计、加强专业人员培训及提升患者参与度等策略,进一步提升ICBT的应用效果,并推动其在青少年群体中的广泛应用。Objective: This study aims to review the application and efficacy of Internet-Based Cognitive Behavioral Therapy (ICBT) in adolescents with anxiety and depression, providing theoretical support for its wider use in China. Methods: A literature review was conducted to analyze the current state, efficacy, and advantages of ICBT for adolescent anxiety and depression. The focus was placed on three intervention models: self-guided ICBT, therapist-supported ICBT, and blended ICBT, highlighting their characteristics and challenges. Results: The analysis revealed that ICBT effectively reduces anxiety and depression symptoms in adolescents. It offers advantages such as improving access to mental health services, reducing social stigma, and providing personalized support. However, challenges remain regarding poor adherence, unclear long-term outcomes, and cultural adaptation, which require further study and optimization. Conclusion: ICBT is an effective intervention for adolescent anxiety and depression with significant potential for broader application. Future efforts should focus on optimizing ICBT platform design, enhancing professional training, and increasing patient engagement to improve its efficacy and facilitate its widespread use among adolescents.