摘要：在教育部高等学校课程思政建设要求下,结合环境工程专业人才培养目标和课程特点,开展环境工程微生物学实验马克思主义理论课程思政,探索思政建设融合模式和实施路径。通过将马克思主义理论融入教学内容从而发挥育人作用、以及用马克思主义观点方法指导学生学习方法学习态度,使专业教学与思想教育深度融合。具体的模式是:将环境工程微生物学实验的教学内容分成几个模块,不同的模块可以分别融入马克思主义科学自然观、马克思主义科学实践观和马克思主义科学幸福观等思政元素;同时运用马克思主义的观点方法来指导学生观察问题和分析问题,培养学生严谨的学习态度和解决问题的能力。环境工程微生物学实验马克思主义理论课程思政的实施,提高了学生的思想水平、专业学习效率和实践能力,有利于高校高素质人才的培养。Under the requirements of ideological and political construction of higher education of the Ministry of Education, combined with the training objectives and curriculum characteristics of environmental engineering majors, the ideological and political courses of environmental engineering microbiology experiment are carried out, and the integration mode and implementation path of ideological and political construction are explored. By integrating Marxist theory into the teaching content so as to play the role of education, and guiding students’ learning methods with Marxist viewpoints and methods, professional teaching and ideological education are deeply integrated. The specific model is to divide the teaching content of environmental engineering microbiology experiments into several modules, each of which can be integrated with ideological and political elements such as the Marxist scientific view of nature, the Marxist scientific view of practice, and the Marxist scientific view of happiness. At the same time, the Marxist perspective and methodology are applied to guide students in observing and analyzing problems, cultivating their rigorous learning attitude and problem-solving skills. The implementation of ideological and political courses of Marxist theory has improved students’ ideological level, professional learning efficiency and practical ability, which is conducive to the cultivation of high-quality talents in colleges and universities.