摘要：[目的]在核DNA水平根据DNA序列变异分析我国沿海8个互花米草种群的遗传结构。[方法]根据互花米草EST序列EH277045的序列信息设计引物,在8个种群共75份样本中扩增该序列,采取直接测序的方法,比较序列差异,计算多种遗传参数,分析中国互花米草种群的遗传多样性与变异。[结果]共得到28个多态位点,将75份样本分为25种单倍型,核苷酸多样性为0.011,单倍型多样性为0.794;种群间基因分化系数GST为0.163,遗传分化指数FST为0.222;AMOVA分析揭示79%的遗传差异来源于种群内,21%的遗传差异来源于种群间。[结论]我国互花米草种群遗传多样性水平高,种群间遗传分化较低,遗传多样性主要存在于种群内。

摘要：以狼尾草和聚乙烯塑料为原料,采用正交实验设计的方法研究了狼尾草的草粉加量、草粉尺寸、相容剂(PE-MAH)等工艺参数的变化条件对复合材料性能的影响,研究结果表明:PE-MAH可以增加草粉与PE的界面结合力,提高复合材料的性能;增加狼尾草的草粉含量可以提高复合材料的拉伸弹性模量、弯曲性能,但拉伸强度和耐水性能有所降低。

摘要：[Objective] This study aimed to analyze the genetic structure of eight populations of Spartina alterniflora in China at the nuclear DNA level.[Method] The EH277045-derived sequences were amplified from 75 samples in 8 populations and directly *** diversity,haplotype diversity,the mean value of Nei＇s genetic distance,genetic differentiation index FST and other genetic parameters were calculated to estimate the genetic diversity and genetic differentiation of *** populations in China.[Result] 75 samples were divided into 25 haplotypes by 28 polymorphic *** high nucleotide diversity（π=0.011） and haplotype diversity（Hd=0.794） were *** mean value of Nei＇s genetic distance and genetic differentiation index FST among eight populations were 0.056 and 0.222,respectively,the Nei＇s genetic distance ranged from 0.000 to 0.189 and FST ranged from 0.000 to 0.444 between each pair of the eight *** result revealed that 79% and 21% of the total genetic variation was partitioned within and among *** populations,respectively.[Conclusion] At the nuclear DNA level,there were a relatively high level of genetic diversity and a relatively low level of genetic differentiation among *** populations in China,and the genetic diversity existed mainly within rather than among populations.

摘要：从象草中克隆了CpCCoAOMT2基因,并对其进行了初步的生物信息学分析。利用转录组测序获得的表达序列信息,设计1对特异性的PCR引物,应用RT-PCR技术从象草中扩增出CpCCoAOMT2基因。对获得的基因进行序列同源性搜索、结构域搜索及3D结构预测、分子系统进化树构建等生物信息学分析。测序结果表明,CCoAOMT2基因全长926 bp,含110 bp的5'-UTR、741 bp的CDS和75 bp的3'-UTR,共编码246个氨基酸,Gen Bank登录号为Bank It1975804;Blastn和Blast T分析结果显示,CpCCoAOMT2基因及其编码的蛋白在核苷酸水平和氨基酸水平上与多种植物的CCoAOMT基因和CCoAOMT蛋白具同源性;预测CpCCoAOMT2蛋白相对分子质量为27 253,等电点为5.08,是一个不含跨膜结构域、不含信号肽分子的亲水性、稳定蛋白。分子进化树分析结果表明,CpCCoAOMT2蛋白与禾本科植物的CCoAOMT蛋白亲缘关系最近,与裸子植物和蕨类植物关系较近,而与双子叶植物的关系较远。