摘要：对以弱碱性大孔阴离子树脂二乙胺基乙基羟乙酯(DEAE-E/H)为载体制备得到的固定化氨基酰化酶的性质进行系统研究。结果表明,DEAE-E/H固定化氨基酰化酶具有很好的热稳定性。与自由酶相比,固定化酶的最适反应温度分别由47℃变为57℃,pH值由7.0变为6.5,并且最适反应条件范围明显变宽。Co2+对固定化酶活力具有较强的激活作用,其最佳浓度为1×10-3mol/L。分别对其在间歇和连续操作过程巾的反应进行研究,结果表明,固定化酶具有较高的操作稳定性。

摘要：在多级串联的φ40×1450mm离子交换柱中进行了001×7NH_4^+型阳离子交换树脂吸附谷氨酸的研究。测定了不同床高下液、固相谷氨酸浓度和pH值的分布。研究了液速、料液谷氨酸浓度对交换区高度的影响,确定了吸附率不小于95%的实际操作条件范围内的最大交换区高度为6.08m。首次测定了不同pH、一定NH_4^+浓度条件下的吸附平衡数据,并进行了关联,确定了一定条件下离子交换柱的理论级数。该工作为过程和设备的放大设计提供了初步依据。

摘要：Aminoacylase was immobilized on the mycelium cells of Aspergillus oryzae by using ethylenediamine, gelatin and glutylalhyde. The proper immobilized condition was studied by orthogonal experimental design. The immobilized cells with excellent activity and stability for optically resoluting N acetyl DL alanine were obtained. The effects of pH, temperature, ion concentration and substrate concentration on the reactive activity of immobilized cells were studied. The continuous optical resolution of N acetyl DL alanine was investigated respectively in an immobilized cells column (ICC) and in a novel couple immobilized cells bed & membrane reactor(CICBMR). The results indicate that the immobilized cells are suitable for industrial applications.