摘要：根据基因库中黄瓜花叶病毒(Cucumber mosaic virus,CMV)、百合斑驳病毒(Lily mottle virus,LMoV)、百合无症病毒(Lily symptomless virus,LSV)的外壳蛋白基因序列,设计了3对特异引物,通过对扩增条件进行优化,建立了同时检测CMV、LMoV和LSV的多重RT-PCR检测方法。该方法可以从带有CMV、LMoV和LSV的样品中同时扩增出3条大小与试验设计相符的657bp(CMV)、428bp(LMoV)、171bp(LSV)的特异性多重RT-PCR扩增带。扩增产物测序表明,CMV、LMoV和LSV三种病毒与GenBank中登录的亚洲和荷兰多数分离物核苷酸同源性在90%以上,地域差异不明显,外壳蛋白序列高度保守。

摘要：依据非酒精性脂肪肝的诊疗指南和医学进展,初步探讨中药治疗非酒精性脂肪肝的Ⅱ期临床研究的目的、试验总体设计、诊断标准及影响因素、治疗方案、观察项目、观察时点、质量控制、疗效评定、统计分析等技术要点,为其临床研究方案设计提供参考。

摘要：[Objective] The effects of different matrix formulations and NAA concentra-tions on the growth of roots and survival rate of rosemary cuttings were investigat-ed. [Method] The randomized block design was adopted. There were 9 formulations of matrix and 4 concentrations of NAA in the test. The survey and determination data was analyzed by Excel. [Result] For rosemary cuttings, the roots of Treatment 7 （turfy soil∶perlite=3∶2） had the best growth with the highest survival rate of nearly 100%. The roots of rosemary cuttings had the best growth for 150 mg/L of NAA with the highest survival rate of 88.9%. [Conclusion] For rosemary cuttings, the op-timal matrix formulation was turfy soil∶perlite=3∶2, and the optimal NAA concentration was 150 mg/L.