摘要：目的:为提高CpG基序重组菌的发酵水平,降低CpG重组质粒的生产成本,运用响应面法优化CpG重组菌发酵培养基。方法:利用Plackett-Burman试验筛选出影响发酵水平的3个最显著因素:糖蜜、玉米浆和工业蛋白胨。然后用响应面设计试验并优化得到最显著因素的最佳浓度,并进一步通过发酵试验验证优化后的培养基。结果:得到一组具有较高菌浓及质粒产量且价格低廉的发酵用培养基:糖蜜4.5g/L,玉米浆8.5g/L,工业蛋白胨8.5g/L,甘油10mL/L,KH2PO41.5g/L,K2HPO42.3g/L,MgSO4.7H2O 0.25g/L,在此培养条件下,OD600实测值为0.6771,理论值为0.6643,两者接近,与标准LB培养基相比,质粒产量提高了15%左右。结论:最终筛选到的培养基具有较高的性价比。

摘要：This report describes the design and synthesis of gold nanostars(AuNSs) containing liposomes by the in situ reduction of gold precursor,HAuCU(pre-encapsulated within the liposomes) through HEPES diffusion and *** with the conventional process that encapsulates the pre-synthesized gold nanoparticles into liposomes during the thin-film hydration step,this facile and convenient method allows the formation and simultaneous encapsulation of AuNSs within *** absorption spectra of AuNSs can be tuned between visible and near infra-red(NIR) regions by controlling the size and morphology of AuNSs through varying the concentrations of HAuCU and *** a proof of concept,we demonstrate the synthesis of AuNSs with a maximum absorbance at 803 nm within the temperature-sensitive *** liposomes can produce stronger photoacoustic signals(1.5 fold) in the NIR region than ***,when there are drugs(i.e.,doxorubicin) within these liposomes,the irradiation with the NIR pulse laser will disrupt the liposomes and trigger the 100%release of these pre-encapsulated drugs within 10 *** comparison,there is neglectable contrast enhancement or minor release(10%) of drugs for the pure liposomes under the same ***,cell experiment shows the potential therapeutic application of this system.