摘要：研究选用优质杂交油菜新组合湘杂油２号进行四元二次回归旋转组合设计栽培试验，将种植密度、施氮量、施磷量、施钾量四种对油菜产量形成影响较大的农艺因素作为决策变量，产量作为目标函数，建立数学预测模型，通过分析各因素及因素间交互效应对产量的影响，利用计算机模拟寻优，提出了亩产达到１５０ｋｇ以上的高产栽培优化区间，即每亩种植密度１．１３—１．４０万株，施Ｎ量１９—１９．８５ｋｇ，施Ｐ２Ｏ５量７．５８—８．５５ｋｇ，施Ｋ２Ｏ量６．０１—７．７７ｋｇ。

摘要：[Objective] Ogura cytoplasmic male sterility （Ogu CMS） is an ideal pollina- tion control system for heterosis utilization in Brassica napus. However, fertility-restor- ing （Rf） gene only exists in radish chromosome and is closely linked with glucosino- late gene, making it hardly to be applied directly in production. Thus, the key to apply Ogu CMS in Brassica napus is to introduce the Rf genes and to break its linkage with glucosinolate gene. [Me^od] To overcome the interspecific reproductive barrier, grafting was conducted by using Raphanobrassica （2n=-58） as donor materi- als of Rf genes. The obtained interspecific hybrids were analyzed from the agronom- ic traits, seed-setting rate and fertility restoration rate for screening fertility-restoring materials. [Results] By elaborative selection, a homozygous Ogu CMS fertility-restor- ing B. napus material named CLR650 was selected out, whose somatic chromosome numbers were found to be 2n=38-40. Some abnormal phenomena like anaphase bridges and lagging chromosomes in meiosis were observed, but the abnormalities did not affect the formation of normal pollens. It can restore the male fertility in both progenies of self-pollination and testcross by 100%. Molecular analysis showed that CLR650 harbors Ogu CMS fertility-restoring gene, which is obviously different from that of Ogu CMS restore line Rl13 and RHH1 by detecting the molecular markers closely linked with radish restorer gene （Rf0）. [Conclusion] The CLR650 could be a new restorer for the Ogu CMS in B. napus.