摘要：目的调查海口市犬肠道内寄生虫——犬复孔绦虫的流行情况,并研究不同生物染色剂对犬复孔绦虫虫体的染色效果,确定最佳染色方法作为虫种形态学鉴别依据。方法使用正交试验设计法,选择醋酸卡红、齐尔石碳酸复红、德氏苏木素、孔雀绿及盐酸卡红五种不同的生物染色剂对虫体节片在不同时间、不同浓度分别染色,并对这五种生物染色剂两两组合进行复合染色,光学显微镜下观察形态结构并鉴别虫种。二甲苯透明法和冬青油透明法处理虫体节片,和染色法进行效果比较。结果 20%醋酸卡红染色12 h、24 h和10%醋酸卡红染色24 h时染色成节效果更佳;齐尔石碳酸复红染液在48 h、72 h染色成节效果最佳;德氏苏木素染液在8~20 h时染色孕节效果最佳;4%的孔雀绿在6 h时染色孕节效果最佳;盐酸卡红在4~8 h时染色孕节效果最佳;复合染色时石碳酸首染,孔雀绿复染效果最佳。透明法处理孕节片效果佳。结论选择合适染色剂及确定最佳染色方案,可清晰观察到绦虫节片的形态结构,有利于虫种鉴别和病原学诊断。

摘要：Covalent organic frameworks(COFs)are garnering significant interest in photocatalytic CO_(2) ***,their limited efficiency in separating photogenerated carriers and the scarcity of catalytic sites lead to suboptimal photocatalytic *** we develop a molecular engineering approach to design a pyrene-based COF(PyTa-H COF)confined cobalt single atoms photocatalyst(Co SACs)for CO_(2) *** moiety is introduced to enhance visible-light harvesting capability and improve charge separation in the ***,Co SACs enhance the photocatalytic activity by accelerating the migration of photo-induced carriers and reducing the reaction energy of the rate-determining ***,PyTa-H@Co achieves a CO production rate of 18.36 mmol g^(−1) h^(−1) and a selectivity of 94%in 4 h under visible light irradiation,which is comparable to the reported best-performing *** functional theory calculations reveal that the Co SACs greatly stabilize*COOH and significantly reduce the energy of the decisive step,leading to outstanding photocatalytic *** work,through molecular engineering design,highlights the critical relationship between catalyst structure and function in enhancing photocatalytic efficiency.