摘要：为了能快速鉴别猪伪狂犬病病毒流行株,根据猪伪狂犬病病毒gE基因前半段95 bp~210 bp区域处设计一对特异性引物及探针,优化反应条件后,建立一种可鉴别猪伪狂犬病病毒gE缺失疫苗株与流行株的Taqman荧光定量PCR检测方法。结果显示,该方法对pMD-PRV gE阳性标准质粒的最低检出下限为4.4×10^(1)拷贝/μL;该方法特异性强,对猪常见病毒性疾病(猪瘟病毒、猪繁殖与呼吸综合征病毒、猪圆环病毒2型等)均无交叉反应,将PRV流行株和疫苗株(Bartha-K61株、HB2000株、C株、Ea株)同时与某商品化试剂盒对比检测,特异性优于某商品化荧光定量PCR检测试剂盒;组内和组间重复性试验的变异系数均小于1.6%,重复性好。应用本研究建立的荧光定量PCR检测方法对165份临床样品进行检测,阳性率为2.42%(4/165),选取已知3份阳性的30份临床样品,与商品化检测试剂盒比对,符合率100%。因此,本研究建立的PRV gE基因荧光定量PCR检测方法可用于猪伪狂犬病病毒流行株的快速诊断和流行病学调查,为PR的控制与净化提供技术支持。

摘要：Methanol to olefins(MTO)reaction as an important non-oil route to produce light olefins has been industrialized,and received over 80% ethylene plus propylene ***,to achieve high single ethylene or propylene selectivity towards the fluctuated market demand is still full of ***-pore SAPO-14 molecular sieve is a rare MTO catalyst exhibiting extra-high propylene *** provides us a valuable clue for further understanding of the relationship between molecular sieve structure and MTO catalytic *** this work,a seconds-level sampling fixed-bed reactor was used to capture real-time product distributions,which help to achieve more selectivity data in response to very short catalytic life of *** in product distribution,especially during the low activity stage,reflect valuable information on the reaction *** with in situ diffuse reflectance infrared Fourier-transform spectroscopy,in situ ultraviolet Raman measurements and ^(12)C/^(13)C isotopic switch experiments,a reaction pathway evolution from dual cycle to olefins-based cycle dominant was *** addition,the deactivation behaviors of SAPO-14 were also investigated,which revealed that polymethylbenzenes have been the deactivated species in such a *** work provides helpful hints on the development of characteristic methanol to propylene(MTP)catalysts.