摘要：九华山是我国四大佛教圣地,历史文化源远流长,佛教文化博大精深;自然风光秀丽,生态植被良好;自然环境、人文环境、地理环境优越;气候宜人,空气清新。九华山老田山庄是五星级生态度假山庄,位于九华山风景名胜区柯村河西老田地段,总占地面积250亩,是集当地民风、民俗文化和园林生态于一体的高档主题性酒店。文章主要介绍了九华山老田山庄建筑规划设计过程。

摘要：在中国,随着城市化的进程日益加快的同时,也迎来了老龄社会的到来。中国的基本国情决定了中国老龄化问题比其他发展中国家更令人担忧。目前很多问题摆在我们面前亟待解决,其中老年人居住建筑的舒适性和安全性就是一个突出的问题。文章从现有住宅适老改造的必要性、原则、内容以及实施等几个方面提出了自己的思考和探讨。

摘要：Rice cultivar Norin 8 and its mutant Norin 8m harbour bentazon resistance trait and bentazon susceptibility trait respectively. A total of 360 arbitrary 10-mer oligonucleotide primers were screened on the genomic DNA of Norin 8 and Norin 8m with RAPD technique. Among which, five primers produced seven polymorphic RAPD bands between Norin 8 and Norin 8m. Amplified RAPD polymorphic products were cloned and sequenced. The sequences were used to design primers for PCR. Five SCAR markers, SCAR/G18/883, SCAR/G18/890, SCAR/G18/919/948, SCAR/D10/1237 and SCAR/F03/1186, were developed from OPG18/943, OPG18/972, OPD10/1248 and OPF03/1198. F-2 progeny of 320 individuals was analyzed to map SCAR markers in relationship to ben or Ben genes. SCAR markers of SCAR/G18/883, SCAR/G18/890, SCAR/G18/919/948 were shown to cosegregate with ben or Ben genes, and SCAR/D10/1237 to be linked of Ben gene with a distance of (14.8 +/- 2.1) cM. The genetic linkage to ben gene and SCAR markers was identified by a pair of near isogenic lines H121 and Hben121. Southern blotting analysis and segregation ratio of F-2 progeny revealed that OPG18/943 and OPG18/972 were single-copy in genome, and locus of OPG18/943 and OPG18/972 were allelic and sequence tagged sites. It is the first report on molecular markers linked to ben or Ben genes. The markers are useful to marker-assisted selection for the breeding and tag ben gene with map-based cloning.

摘要：本研究建立了转基因耐除草剂大豆A2704-12定性PCR检测方法。根据A2704-12转化体外源插入片段5'端与大豆基因组连接区序列设计特异性引物,以大豆内源基因Lectin为内标,从A2704-12转化体中特异性扩增出239bp大小的特异性目的片段。同时对该方法特异性和灵敏度进行测试,结果显示:该方法能检测出A2704-12转化体中的特异性DNA片段;其检测灵敏度可达到0.05%。本文建立的方法特异性强,灵敏度高,可为今后转基因耐除草剂大豆A2704-12的市场监管提供技术支撑。