摘要：在应用小鼠胚胎干细胞(ESCs)裂解液诱导异性小鼠胚胎成纤维细胞(MEFs)为诱导性多能干细胞(iPSCs)的过程中,通过性别鉴定的方法筛选特定性别的被诱导的MEFs,能够快速简易的鉴定出iPSCs的生成材料是否来源于被诱导的体细胞。利用妊娠13.5天雄性和雌性胎鼠生殖嵴在形态学上存在明显差异的特性,识别出不同性别昆明系小鼠13.5天的胚胎,并从中分离培养出大量不同性别的MEFs,用于iPSCs的实验研究。根据雄性小鼠特异性的Y染色体性别决定区(SRY基因)的核心序列,设计一对引物,并对获得的不同性别MEFs的DNA样本进行PCR扩增,验证不同性别胎鼠生殖嵴在形态学上存在的差异。结果表明,PCR性别鉴定结果与MEFs来源胚胎的性别一致。不同性别的MEFs均具有典型的成纤维细胞特性,两者在形态学上并没有差异。因此,利用雄性与雌性胚胎在生殖嵴形态学上的差异可以快速分离获得大量不同性别的MEFs。

摘要：Actinide-based catalysts have been regarded as promising candidates for N_(2) fixation owing to their unique 5f orbital with flexible oxidation ***,we report for the first time the dispersion of uranium(U)single atoms on TiO_(2) nanosheets via oxygen vacancy confinement for N_(2) *** single-atom U catalyst exhibited a high NH_(3) yield of 40.57μg h^(-1) mg^(-1),with a reasonably high Faraday efficiency of 25.77%,ranking first among the reported nitrogen-free ***-labeling operando synchrotron infrared spectroscopy verifies that the key*N_(2)H_(y) intermediate species was derived from the N_(2) gas of the *** using operando X-ray absorption spectroscopy,we found enhanced metal-support interaction between U single atoms and TiO_(2) lattice with more U-O_(latt) coordination under working *** simulations suggest that the evolved 1O_(ads)-U-4O_(latt) moieties act as a critical electronfeedback center,lowering the thermodynamic energy barrier for the N_(2) dissociation and the first hydrogenation *** work provides the possibility of tailoring the interaction between metal active sites and supports for designing high-performance actinide-based single-atom catalysts.

摘要：Engineering the electronic properties of catalysts to target intermediate adsorption energy as well as harvest high selectivity represents a promising strategy to design advanced electrocatalysts for efficient CO_(2) ***,a synergistical tuning on the electronic structure of the Cd Se nanorods is proposed for boosting electrochemical reduction of CO_(2) .The synergy of Ag doping coupled with Se vacancies tuned the electronic structure of the CdSe nanorods,which shows the metalloid characterization and thereby the accelerated electron transfer of CO_(2) *** synchrotron radiation Fourier transform infrared spectroscopy and theoretical simulation revealed that the Ag doping and Se vacancies accelerated the CO_(2) activation process and lowered the energy barrier for the conversion from CO_(2) to;COOH;as a result,the performance of CO_(2) electroreduction was *** as-obtained metalloid Ag-doped CdSe nanorods exhibited a 2.7-fold increment in current density and 1.9-fold Faradaic efficiency of CO compared with the pristine CdSe nanorod.

摘要：为了实现电力监控系统的移动化、便携化,建立有效的远程电力监控服务体系,设计了基于Android操作系统的电力监控系统移动终端软件,包括用户界面设计模块、实时报警模块、配置文件管理模块、用户管理模块、监控功能模块。实现电站数据查询、远程频主接线图的查询、变电站设备的远程控制以及报警事件推送和软件用户管理等。

摘要：为筛选适宜高温高湿气候的奶牛养殖种群,本研究对荷斯坦奶牛、娟姗牛、娟荷杂交一代奶牛进行了耐热生理、血清生化指标的测定。结果表明:在持续热应激刺激下奶牛的直肠温度、呼吸频率均显著增加(P娟杂牛组>荷斯坦牛组,HSP70的上升幅度为娟杂牛组>娟姗牛组>荷斯坦牛组;热应激使T_3、T_4及SOD的含量显著下降(P<0.05),其中T_3、T_4下降幅度最大的均为娟杂牛组(66.10%和42.93%),而SOD下降幅度最大的为荷斯坦牛组(49.76%)。综上所述,在热应激条件下,娟姗牛的生理指标适应度优于娟杂牛和荷斯坦牛;在抗病能力及热适应性的生化指标方面娟姗牛和娟杂牛均优于荷斯坦牛;在降低热增耗及HSP70表达量方面娟杂牛优于娟姗牛和荷斯坦牛。

摘要：通过分析变电站屏顶小母线的结构和施工过程,提出了一种低成本的屏顶小母线连接器与监控模块设计方案,并设计了产品外观,分析了该设计的优缺点及未来的改进方案。该设计方案降低了在变电站屏柜施工过程中小母线搭接的施工风险和难度,推动了变电站屏顶小母线施工方案的发展,补齐了小母线拆接过程中发生故障的短板。

摘要：目的:调查浙江省社区低龄老人对“小老顾老老”及“时间银行”新型养老模式的接受程度,为探究老年人居家养老问题解决方案提供新方向.方法:采用便利抽样法选取浙江省可及社区低龄老人(60~75岁)389例,应用自行设计的相关问卷进行实地走访调查.结果:问卷应答率为96.7%,73.6%低龄老人可接受“小老顾老老”即照顾高龄老人,其中52.8%低龄老人愿意提供“无偿”照护,36.1%低龄老人有“时间银行”储蓄的意愿.结论:以“时间银行”为基础的“小老顾老老”的新型养老互助模式得到了低龄老年人较为广泛的认同,具有一定可行性.但未来仍需加强顶层设计,完善监管体系,提升服务质量,加大宣传力度,促使互助养老和尊老爱老蔚然成风.