摘要：新城疫、禽传染性支气管炎与禽流感是可引起禽类呼吸道症状相似的三种急性传染病,且存在混合感染。为建立新城疫病毒、禽传染性支气管炎病毒与禽流感病毒三重RT-qPCR检测方法,该研究对比了GenBank登录的新城疫病毒(NDV)L基因、禽传染性支气管炎病毒(IBV)1a基因和禽流感病毒(AIV)NP基因,经分析选取保守序列设计了三套引物与探针,通过优化检测方法的反应体系,成功建立了可同时检测NDV、IBV和AIV的三重RT-qPCR方法,并对其特异性、敏感性和重复性进行了评估。结果表明,该方法能够特异性检测出NDV、IBV和AIV,有较好的特异性;方法对三种病毒的最低检测限均为3.02×10^(2)copies/μL,有较高的敏感性;重复性实验结果显示批内与批间变异系数均不大于1.28%,有良好的重复性。使用本研究建立的方法对102份临床咽拭子进行检测,并与常规RT-PCR进行比较,总符合率为96.6%。本研究建立的三重RT-qPCR检测方法,特异性强,灵敏度高,可用于NDV、IBV、AIV三种病毒的检测。

摘要：汽车座椅骨架的优化设计与研究,采用了一款汽车标配的主驾驶座椅作为研究目标,先是考虑了座椅的安全性能,测量了原座椅的数据,使用建模软件SolidWorks建立了适合有限元分析的简化模型,并基于国家对汽车座椅的相关安全标准,提出了适合本文使用的研究和优化方案:首先,使用ANSYS对座椅骨架进行最基础的静力学分析,在保证其结果满足国家标准后模拟实际工况对其进行疲劳分析,结合静力学分析和疲劳分析的结果对座椅骨架最薄弱的部分展开优化设计,其次对模型进行模态分析,保证座椅试验不会产生共振。本文的优化主要采用了尺寸优化与轻量化,对优化后的模型进行相同工况的试验并于优化前进行对比,来证实本文优化设计的可行性。The optimization design and research of car seat frames adopted a standard driver’s seat as the research objective. Firstly, the safety performance of the seat was considered, and the data of the original seat was measured. A simplified model suitable for finite element analysis was established using the modeling software SolidWorks. Based on the relevant national safety standards for car seats, a research and optimization plan suitable for this article was proposed. Firstly, ANSYS was used to conduct the most basic static analysis of the seat frame. After ensuring that the results meet the national standards, fatigue analysis was simulated under actual working conditions. Combined with the results of static and fatigue analysis, the weakest part of the seat frame was optimized. Secondly, modal analysis was carried out on the model to ensure that resonance would not occur in the seat test. The optimization of this article mainly adopts size optimization and lightweighting. The optimized model is tested under the same operating conditions and compared before optimization to confirm the feasibility of the optimized design in this article.

摘要：为了实现对车辆尾号的限行管理,采用虚拟仪器技术和紧凑型视觉系统,通过电荷耦合型(CCD)摄像机获得车牌的图像,应用LabVIEW软件对图像进行阈值处理、数字滤波、车牌定位、字符分割、字符识别以及人机交互界面设计,实现了车牌尾号自动识别,并能自动进行车牌尾号限行判断,实现了车牌尾号自动识别系统。该系统识别判断正确率达90%,表明该自动识别系统可行。

摘要：文章以南昌地铁某盾构区间隧道切削桥梁单桩基础为背景,通过数据分析,介绍了泥水盾构切削大直径桥桩的施工控制参数。分析结果表明,切桩时宜调低刀盘转速和推进速度,降低刀盘扭矩和推力;宜上调泥浆比重,下调黏度。为防止切削下的桩体钢筋影响刀盘正常转动,宜在盾构超过混凝土保护层适当距离后,停机进仓检查或清理一次。

摘要：近年来,虽然汽车技术在不断发展,但控制类汽车电子技术的发展却进步较为缓慢,从而导致国内汽车产业在电子技术方面存在一些短板。本研究旨在设计并实现一套基于语音识别的汽车灯光控制系统,以提高驾驶的安全性和操作的便捷性。同时,现有的汽车电子控制系统多依赖于国外的技术,这不仅限制了国内技术的发展,也影响了汽车产业的自主创新能力。本文对语音识别系统进行设计研究,并对比了普通话和方言在语音识别中的准确率。实验结果显示,普通话识别模型准确率高,四川话、广东话和河南话的准确率分别为73.8%、76.2%和59.5%。In recent years, although automotive technology has been continuously developing, the development of control automotive electronic technology has not progressed relatively slowly, resulting in some shortcomings in the domestic automotive industry in terms of electronic technology. This study aims to design and implement a car lighting control system based on speech recognition to improve driving safety and operational convenience. Meanwhile, existing automotive electronic control systems rely heavily on foreign technology, which not only limits the development of domestic technology but also affects the independent innovation capability of the automotive industry. This article conducts design research on speech recognition systems and compares the accuracy of Mandarin and dialects in speech recognition. The experimental results show that the Mandarin recognition model has high accuracy, with accuracy rates of 73.8%, 76.2%, and 59.5% for Sichuan dialect, Cantonese, and Henan dialect, respectively.

摘要：接管相贯区域的应力分析设计是压力容器强度研究领域的重要方面。根据工程实践中的技术需求,本文采用有限元方法对圆柱形壳体与非径向齐平式接管的相贯区域受力特性进行了研究。研究发现,首先,与接管载荷相比,内压是结构受力的主导因素;其次,应力集中点应力值随接管倾斜角先轻微增大然后减小;再次,等效线性化处理后得到的局部薄膜计算值随接管倾斜角单调减小。总体而言,接管倾斜角对相贯区域受力无不利影响。