摘要：Found on the hill on the northern bank of the Yujinag River in the territory of Zhongjiang County,Sichuan,nine of the cliff tombs underwent rescue excavation in2002from September to November. Each tomb consisted of a tomb passage,a tomb gate,a main chamber,a tunnel,a side cham-ber,a coffin bed,a wall recession and a *** tombs are either single chambered,double-chambered or *** paintings with ink caption were found in cliff tombs for the first *** addition,discovery of carved figurines and sculptures in the form of some wooden ar-chitecture enriches largely our knowledge of cliff *** tombs should be dated back to the middle and late Eastern Han dynasty.

摘要：本文按水蛭素HV2的氨基酸序列,设计并合成了水蛭素基因,并在酵母α因子表达系统中表达。通过诱变得到的稳定携带水蛭素表达质粒的酵母菌株,在丰富培养基中培养36—48 h后,可在培养液中测得10—20 ATU/ml的分泌的水蛭素表达产物。经过较简单的纯化步骤,得到HPLC纯的水蛭素,其N-端氨基酸序列与天然产物完全相同,并有强烈的抗凝血和抑制凝血酶的活力。从500 ml培养液中可得到约3000 ATU的纯水蛭素,其比活力为6600 ATU/mg。