摘要：本文采用二因子回归最优设计的方法,研究了夏谷高产与施纯氮量、P_20_5量之间的关系,建立了夏谷产量与两项农艺措施的函数模型;通过数学模拟筛选出了谷子亩产400kg以上的最佳农艺措施组合方案。

摘要：为了从分子水平上鉴别不同的转基因株系,以转BADH基因苜蓿的T0代基因组DNA为模板,采用热不对称交错PCR(TAIL-PCR)方法分离其外源基因插入位点的侧翼序列,获得了B127株系的左翼序列和右翼序列,以及B125、B138、B295和B196株系的左翼序列。侧翼序列特征分析表明,有的T-DNA边界序列被删除,有的边界序列被保留,并填充了一段未知来源的核苷酸序列。根据侧翼序列中插入载体序列和紧邻插入序列的基因组序列特征,分别设计PCR扩增的上、下游引物,并对获得的42个转BADH株系分别进行左、右翼序列的扩增,结果表明,转基因植株B106、B125、B138、B157、B158、B289、B295、B305和B127具有相同的扩增条带,B203、B220、B223和B196具有相同的扩增条带,说明这些株系可能仅来源于2个转化事件。本研究建立的事件特异性检测方法可以准确地将不同的转化株系区别开来。

摘要：[Objective] This study aimed to investigate the safe dosage of imazethapyr for an imazethapyr resistant millet variety and its effect on weed control. [Method] The Imazethapyr resistant millet ＂M1508＂ was used as the experimental material and the experiment was arranged in a split plot design with two factors： herbicide application time and herbicide dosage. Application time was taken as the main plot with six treatments： A1 （preemergence treatment）, A2 （postemergence treatment on the 3rd d）, A3 （postemergence treatment on the 6th d）, A4 （postemergence treatment on the 9th d）, A5 （postemergence treatment on the 12th d）, A6 （postemergence treatment on the 15th d）; spraying dosage（B） with nine treatments： B1 （0 ml/hm2 , taking water as control）, B2 （750 ml/hm2 ）, B3 （1 125 ml/hm2 ）, B4 （1 500 ml/hm2 ）, B5 （2 250 ml/hm2 ）, B6 （3 000 ml/hm2 ）, B7 （4 500 ml/hm2 ）, B8 （6 000 ml/hm2）, B9 （7 500 ml/hm2 ）; water was added at 675 kg/hm2 . [Result] Imazethapyr showed a good herbicidal effect, and the imazethapyr resistant new variety M1508 also had a high drug resistance; the dosage range of 750 -7 500 ml/hm2 was safe for millet; considering the weed control efficacy, the yield and weeding cost comprehensively, postemergence treatment on the 6th day at 1 500 -2 250 ml/hm2 was the best for herbicide application. [Conclusion] Matched chemical weeding techniques to imazethapyr resistant millet varieties were preliminarily identified, and the efficacy of imazethapyr on weed control and its security on millet were investigated, providing references for the application and promotion of imazethapyr resistant millet varieties.