摘要：筛选117条炭疽芽胞杆菌（Bacillus anthracis）特异序列，经双重特异性验证后得到19条理想的特异序列（genomic signatures），其中6条符合设计TaqMan探针建立实时定量PCR的要求，根据常规PCR检测结果选择其中CO4片段与炭疽芽胞杆菌毒性质粒pX01、pX02上的pagA、capB基因建立实时定量PCR检测体系。经试验证实这一体系检测灵敏度达到每PCR反应10～100个拷贝。利用12种相关菌株评价后获得100％特异性，对10份模拟污染标本和20份对照标本检测，所有污染标本均被检出，所有对照标本均为阴性。此方法特异、灵敏、高效，在炭疽芽胞杆菌感染的诊断和环境污染的检测等领域有潜在的应用前景。

摘要：Shanxi Hua tong Neon Light Decoration Company is the member of China Neon Light Association. It designs makes and install all kinds of neon light advertisement billboard and light boxes for customers. Their products are so popular owing to the advanced EGL technology imported from US, the widely using of computer controlling and programming, the better materials from foreign countries. The use of vacuum molecular pump FB - 1 10 - 2 makes the company the first in China to apply the most advance space - night instrument in neon light industry.