摘要：采用平板初筛和摇瓶复筛等方法,从秦皇岛渤海海域的海水样品中,分离获得产淀粉酶活力较高的菌株HYM-7,初步鉴定为解淀粉类芽孢杆菌(***),设计单因素和正交实验,确定该菌的最佳发酵条件。结果表明:在装液量50mL液体培养基的250mL三角瓶中,采用单因素实验得最佳接种量为1%,最适产酶时间为48h,最适碳源为玉米淀粉,氮源为大豆粉,Ca2+和Mg2+可显著提高产酶量;采用正交实验得最适发酵培养基配方(g/L):玉米淀粉4、大豆粉10、可溶性淀粉2、MgSO40.2、CaCl20.4。对优化的发酵培养基进行验证,测得酶活力为34.62U·mL-1,较基础培养基酶活提高60.8%。

摘要：师范类专业认证以规范引导师范类专业建设,不断提高教师培养质量为核心,是高校提高人才培养规格、持续监控教育质量的重要手段。课程思政是落实高校思想政治教育,实现立德树人根本任务的重要保证。师范类专业认证从根本上与课程思政在目标上同向同行,高度一致。文章以基因工程课程中“限制性内切酶”为例,从内容分析、学情分析、教学目标、方法、设计思路、课堂组织过程、教学总结等方面探索师范认证背景下基因工程课程思政的教学设计。Normal professional certification takes standardized normal majors construction and continuous improvement of the teacher cultivation quality as the core. It is an important means for universities to improve the quality of talent cultivation and continuously monitor educational quality. Curriculum ideological and political education is an important guarantee for implementing ideological and political education in universities and achieving the fundamental task of cultivating morality and talent. Normal professional certification aligns with the goals of curriculum ideological and political education. In the teaching design of genetic engineering course, taking the ‘Restriction Endonucleases’ as an example, the content, learning situation, teaching objectives, methods, design ideas, and classroom organization process were analyzed and explored based on curriculum ideological and political education under the background of normal professional certification.

摘要：采用平板初筛和摇瓶复筛等方法,从渤海秦皇岛海域的海水样品中筛选到产低温蛋白酶活力较高的菌株HYM-16,初步鉴定为黄色杆菌属,设计单因素和正交试验,确定该菌的最佳发酵条件。结果表明,在装液量50 mL液体培养基的250 mL三角瓶中,以体积分数0.01的接种量、摇床转速180 r/min条件下,25℃培养48 h酶活最高;最适碳源、氮源分别为蔗糖、豆饼粉,Mg2+显著提高产酶量;采用L9(34)正交试验得最适发酵培养基配方:蔗糖1 g/L,豆饼粉20 g/L,MgCl2 0.4 g/L,K2HPO4 0.5 g/L,KH2PO4 0.5 g/L。经验证,优化后的发酵培养基菌株酶活达605.8×103 U.L-1,较基础培养基提高了46.9%。