摘要：Objective To establish a rat model of chronic atrophic gastritis and explore the factors inducing *** In accordance with repeated orthogonal design of L8(27), 60% alcohol and 20mmol/L sodium deoxycholate (served as factor A), 0.05%-0.1% ammonia water (factor B), 0.05% indomethacin (factor C) were given, alone or in combination, to rats in three experiments for 3 months, 6 months or 9 months respectively. Then the rats were dissected, and their pathologic changes of the gastric mucosa were *** Typical signs of chronic atrophic gastritis (CAG) were found in all rats which were treated with factor A, B, C alone or in combination for 6 or 9 months. No significant difference of pathologic changes of gastric mucosa was found between the rats treated for 6 months and those for 9 months. No obvious CAG signs were found in the rats treated with factor A, B, C for 3 *** Sixty percent of alcohol, 20mmol/L sodium deoxycholate, 0.05%-0.1% ammonia water and 0.05% indomethacin given to Sprague-Dawley rats for 6 months can successfully establish the animal model of CAG. Prolongation of the model-establishment time is not able to further facilitate the atrophy of gastric mucosa.

摘要：目的　建立鼠慢性萎缩性胃炎 (CAG)动物模型并探讨致萎缩因素。方法　应用① 6 0 %酒精和 2 0mmol/L去氧胆酸钠 (A因素 ) ;② 0 .0 5 %～ 0 .1%氨水 (B因素 ) ;③ 0 .0 5 %吲哚美辛 (C因素 )按有重复的L8(2 7)正交设计对SD大鼠分三批进行制模刺激 ,三批刺激时间分别为 3个月、6个月和 9个月。实验到期后取出全胃 ,观察大鼠胃黏膜大体观及各项萎缩指标。结果　应用A、B、C三因素 (单因素或多因素 )刺激 6个月或 9个月均出现典型的胃窦萎缩性胃炎征象 ,制模成功率达 10 0 % ,且能维持形态稳定 1个月以上。结论　应用 6 0 %酒精和 2 0mmol/L去氧胆酸钠 ,0 .0 5 %～ 0 .1%氨水 ,0 .0 5 %吲哚美辛对SD大鼠刺激 6个月可成功地建立CAG模型 。

摘要：目的 :建立快速鼠慢性胃炎模型 ,并探讨所用的刺激因素对胃粘膜致萎缩的作用。方法 :采用正交设计 ,综合运用 60 %酒精、2 0 mmol/L去氧胆酸钠和 0 .0 5 %氨水刺激 SD大鼠胃粘膜 ,并应用吲哚美辛及低维生素类食物喂饲大鼠 ,1 2周后观察鼠胃粘膜的病理改变。结果 :综合法刺激 1 2周后鼠胃粘膜炎症程度有显著变化 ,胃窦固有腺数明显减少 ,胃体壁细胞数减少 ( P<0 .0 1 ) ;吲哚美辛只引起鼠胃粘膜炎症 ,无萎缩变化。短期低维生素食物无致炎症及萎缩作用 ,且后两者对综合刺激无协同作用。结论 :综合法能建立快速大鼠慢性胃炎模型 。

摘要：本研究采用正交设计和随机分组的方法,分别进行动物及临床试验。采用60％酒精、20mmol/L去氧胆酸钠和0.05％氨水综合刺激SD大鼠胃粘膜,应用非甾体类消炎药(吲哚美辛)及给予低维生素类食物的方法喂饲大鼠,结果表明12周内能快速建立鼠慢性胃炎模型,吲哚美辛对鼠胃粘膜有慢性致炎作用,但较以上综合法刺激致炎作用为弱,而低维生素类食物给予的方法却无致炎作用。观察15例临床上具有酗酒、胆汁返流、Hp感染的男性慢性胃炎患者胃组织活检标本,均较一般慢性胃炎炎症程度明显加重。本研究为探讨慢性胃炎的病因学提供了实验和临床依据。