摘要：In the recent years,engineering new-to-nature CO_(2)-and C1-fixing metabolic pathways made a leap ***,artificial pathways promise higher yields and activity than natural ones like the Calvin-Benson-Bassham(CBB)*** question remains how to best predict their in vivo performance and what actually makes one pathway“better”than *** this context,we explore aerobic carbon fixation pathways by a computational approach and compare them based on their specific activity and yield on methanol,formate,and CO_(2)/H_(2)considering the kinetics and thermodynamics of the *** pathways found in nature or implemented in the laboratory,this included two completely new cycles with favorable features:the reductive citramalyl-CoA cycle and the 2-hydroxyglutarate-reverse tricarboxylic acid cycle.A comprehensive kinetic data set was collected for all enzymes of all pathways,and missing kinetic data were sampled with the Parameter Balancing *** and thermodynamic data were fed to the Enzyme Cost Minimization algorithm to check for respective inconsistencies and calculate pathway-specific *** specific activities of the reductive glycine pathway,the CETCH cycle,and the new reductive citramalyl-CoA cycle were predicted to match the best natural cycles with superior productsubstrate ***,the CBB cycle performed better in terms of activity compared to the alternative pathways than previously *** make an argument that stoichiometric yield is likely not the most important design criterion of the CBB ***,alternative carbon fixation pathways were paretooptimal for specific activity and product-substrate yield in simulations with C1 substrates and CO_(2)/H_(2)and therefore hold great potential for future applications in Industrial Biotechnology and Synthetic Biology.

摘要：In the article titled“In-Depth Computational Analysis of Natural and Artificial Carbon Fixation Pathways”[1],there were errors in Figures 7–*** the label of Figure 9,it reads:“Glyceraldehyde-3-phosphate was chosen as a product for the pathways,”but this is corrected to“Pyruvate was chosen as a product and formate as a substrate for the pathways.”In the lower panels of Figures 7,8,and 10,the labels“MOG/rCCC”and“CETCH cycle”in the figure key should be ***,in these figures,the panel indicators(a)and(b)were missing.