摘要：Many cellular mod.ls have been used.to investigate bone substitutes includ.ng corald.rived.hyd.oxylapatite (HA). The aim of this stud. was to verify whether a new cellular mod.l represented.by Saos-eGFP cells can be used.to test biomaterials by in vitro assays. SaoseGFP cells which express the enhanced.Green Fluorescent Protein (eGFP), were d.rived.from the osteoblast-like cell line Saos-2. To this purpose Saos-eGFP cells were employed.to investigate the in-vitro bioactivity of a well characterized.coral-d.rived.HA biomaterial, in block and.granule forms. This engineered.cell line, by evaluating the emitted.fluorescence, allowed.us to assay (i) cell ad.esion, (ii) cell proliferation and.(iii) colony capability. Electron microscopy analysis was employed.to evaluate the (iv) morphology of cells seed.d.on the biomaterial surface. (v) Histological analysis of the bone grown after scaffold.implantation was carried.out in specimens from two clinical cases. Saos-eGFP cells ind.cate, as established.before, that the coralline-HA biomaterials has a good.in vitro cytocompatibility when tested.with human osteoblast-like cells. Some d.fferences in proliferation activity was d.tected.for the two d.fferent forms assayed. Cytocompatibility d.ta from in vitro analyses were confirmed.by in vivo behaviour of biomaterials. Our tests suggest that the engineered.cell line Saos-eGFP represents a suitable in vitro mod. for stud.ing the biocompatibility, the cell ad.esion, spread.ng and.proliferation on biomaterials d.veloped.for clinical applications. The main ad.antages of this cellular mod.l are (i) less time consuming and.(ii) a red.ced.cost of the experiments.