摘要：Many cells possess the ability to engulf and incorporate particles by *** active process is characteristic of microorganisms as well as higher order *** mammals,monocytes,macrophages,and microglia are among the so-called professional *** addition,cells such as fibroblast and chondrocytes are classified as nonprofessional *** phagocytes play important roles in both the innate and adaptive immune responses,wound healing,and tissue ***,these cells are increasingly studied as targets and vectors of therapeutic intervention to treat a range of *** phagocytes are notoriously difficult to transfect limiting their study and ***,efforts have shifted towards the development of nanoparticles to deliver a cargo to phagocytic cells via ***,this approach carries significant technical challenges,particularly for protein *** have focused on the development of nanoscale cocrystalline protein depots,known as PODS®,that contain protein cargos,including ***,we show that PODS are readily phagocytosed by nonprofessional as well as professional phagocytic cells and have attributes,such as highly sustained release of cargo,that suggest potential utility for the study and exploitation of phagocytic cells for drug *** and macrophages that ingest PODS retain normal characteristics including a robust chemotactic ***,the PODS-cytokine cargo is secreted by the loaded cell at a level sufficient to modulate the behavior of surrounding nonphagocytic *** results presented here demonstrate the potential of PODS nanoparticles as a novel molecular tool for the study and manipulation of phagocytic cells and for the development of Trojan horse immunotherapy strategies to treat cancer and other diseases.