摘要：AIM: To demonstrate the morphology and structure of in vitro reconstructed tissue-engineered human corneal epithelium (TE-HCEP) with seeder cells from an untransfected HCEP cell line. METHODS: The TE-HCEPs were reconstructed in vitro with seeder cells from an untransfected HCEP cell line, and scaffold carriers of denuded amniotic membrane (dAM) in air-liquid interface culture for 3, 5, 7 and 9 days, respectively. The specimens were examined with hematoxylin-eosin (HE) staining of paraffin-section, immunocytochemical staining, scanning and transmission electron microscopy. RESULTS: During in vitro reconstruction of TE-HCEP, HCEP cells formed a 3-4, 6-7 and 8-10 layers of an HCEP-like structure on dAMs in air-liquid interface culture for 3, 5 and 7 days, respectively. But the cells deceased to 5-6 layers and the structure of straified epithelium became loose at day 9. And the cells maintained positive expression of marker proteins (keratin 3 and keratin 12), cell-junction proteins (zonula occludens-1, E-cadherin, connexin 43 and integrin beta 1) and membrane transport protein of Na+-K+ ATPase. The HCEP cells in TE-HCEP were rich in microvilli on apical surface and established numerous cell-cell and cell-dAM junctions at day 5. CONCLUSION: The morphology and structure of the reconstructed TE-HCEP were similar to those of HCEP in vivo. The HCEP cells in the reconstructed TE-HCEP maintained the properties of HCEP cells, including abilities of forming intercellular and cell-extracellular matrix junctions and abilities of performing membrane transportation. The untransfected HCEP cells and dAMs could promisingly be used in reconstruction HCEP equivalent for clinical corneal epithelium transplantation.

摘要：For lessening the weight and volume of flow control system,enlarging the circulation control applying area of angle of attack(AOA),and achieving nice controlling characteristics,a novel lift enhancement method based on dual synthetic jet actuators(DSJAs)and synthetic jet actuator(SJA),and an adaptive proportional integral and differential(PID)algorithm based on radial basis function neural network are *** are uniformly located along the chord to suppress the separation and trailing-edge SJA is applied to achieve the high *** of actuators are modulated to realize the real speed profile and on–off controlling laws of DSJAs are *** simulations show that DSJAs and SJA could suppress the separation completely and move leading-edge stagnation point and trailing-edge separation point downstream even at AOA of 19°,hence achieve the highest lift and nose-down moment augmentation(ΔCl_(max)=0.92,ΔCm_(max)=0.02534),andΔL/D can reach 11.39 at AOA of 18°.Stalling is delayed to more than 19°.Linear lift area and pitch-break angle are both increased to 16°.ΔCl/C_(μ) can reach 76.7,indicating the greatest control *** results of adaptive PID control,whose controlling effects are proved better than PID,indicate that lift could track the objective with the rise time of 0.0325 s and finally keep steady,suggesting the nice stability and rapidness.