摘要：Fusarium crown rot,mainly caused by Fusarium pseudograminearum,is a destructive disease in wheat *** establish a rapid and reliable detection method for ***,the specific PCR primer pair(Fpg-F1;R2)was designed based on the RPB sequence,and real-time fluorescence quantitative PCR(qPCR)was used to validate the efficiency of the *** results showed that the primer pair had high specificity and sensitivity of 100 pg of ***,the qPCR system for early and rapid detection of *** had an amplification efficiency of 87.5%and correlation coefficient of 0.99,and the pathologic threshold of *** in soil was determined by using this detection *** was found that *** could cause Fusarium crown rot when the DNA concentration of *** in field soil exceeded 213 pg·g^(-1).Hence,the qPCR-based method we developed for *** detection has the advantages of high specificity and sensitivity,and can be used for rapid and early detection of *** even in field soils.