摘要：为快速检测猪捷申病毒(PTV),根据PTV 5′UTR基因保守序列设计特异性引物和探针,优化荧光逆转录重组酶介导等温扩增(RT-RAA)反应条件和体系,并通过特异性、敏感性、重复性试验和临床应用对RT-RAA进行评价。结果表明,在37℃恒温反应22 min即可完成对PTV核酸扩增,最低检出限度为30.1 copies/μL;与猪流行性腹泻病毒(PEDV)、猪传染性胃肠炎病毒(TGEV)、猪轮状病毒(PoRV)、猪德尔塔冠状病毒(PDCoV)均无交叉反应;重复性试验显示,组内和组间变异系数均小于5%;23份临床样品检测显示PTV阳性率为21.74%(5/23),检测结果与RT-qPCR一致。说明建立的PTV荧光RT-RAA检测方法具有简便、高效、稳定等优点,为PTV的快速检测和流行病学调查提供了技术手段。

摘要：基于静态流量模式设计的强度调制和直接检测(Intensity Modulation and Direct Detection,IMDD)无源光网络(Passive Optical Networks,PON),无法有效满足新兴业务在带宽、时延和连通性方面的多样化需求。针对这一问题,提出了一种基于级联快速傅里叶逆变换/快速傅里叶变换(Inverse Fast Fourier Transform/Fast Fourier Transform,IFFT/FFT)的点对多点(Point-to-Multipoint,P2MP)光收发机,并在上行IMDD PON系统中进行了实验验证。为了进一步研究该收发机对不同应用场景的适用性,利用上行IMDD PON仿真系统,确定了数字滤波器长度、入纤光功率等最优参数,对该收发机的上行传输性能和灵活性进行了分析。仿真结果表明:在光纤信道衰落不大的情况下,光纤传输距离可以灵活变化,不会明显降低系统传输容量。当信道数≤10时,每个光网络单元(Optical Network Unit,ONU),可以灵活地改变其聚合的信道数,不会显著降低系统传输容量。此外,通过动态改变每个ONU的比特率,灵活地改变其ONU发射功率的动态范围。

摘要：Ferroptosis and autophagy, playing significant roles in tumor treatment, are two typical forms of the programmed cell death. However, the rational combination of ferroptosis and autophagy for synergistic tumor therapy is still highly challenging. Herein, we report on an intriguing nanomedicine strategy for achieving autophagy-enhanced ferroptosis on efficiently combating cancer, which was based on the construction of trehalose-loaded mSiO_(2)@MnO_(x)-mPEG(Tre MMM) nanoparticles with satisfactory biocompatibility. The nanoparticles are endowed with high glutathione(GSH) consumption efficiency, thereby inducing cancer-cell ferroptosis via inactivating glutathione peroxidases 4(GPX4). Subsequently, the Tre MMM degradation due to the GSH depletion and p H sensitivity contributed to the trehalose release for inducing autophagy, promoting/enhancing ferroptosis by NCOA4-mediated degradation of ferritin.A substantial in vitro and in vivo antitumor effect was achieved by such an intriguing autophagyenhanced ferroptosis. Therefore, the rational combination of GSH-consumption-induced ferroptosis and trehalose-induced autophagy by nanomedicine design provides an alternative but effective strategy for tumor treatment.

摘要：A series of novel pyrimidin-4（3H）-one hydroxamate derivatives was designed, synthesized and studied for their activities against histone deacetylases（HDACs）. The results indicate that all the compounds show HDACs inhibitiory activity. The antiproliferative activities of the compounds against HeLa and A549 cells were also investigated. The pharmacological results show compound 9g has potent activity in the enzymatic inhibition assay and cell-based assay.

摘要：旨在制备非洲猪瘟病毒(ASFV)非结构蛋白pS183L的多克隆抗体。根据公布的毒株ASFV China/2018/Anhui XCGQ基因序列设计引物,酶切连接法构建重组质粒pET-28a-S183L;经鉴定成功的质粒转化大肠杆菌Rosetta,诱导表达后经镍柱亲和层析纯化后获得重组蛋白pS183L,免疫小鼠并制备抗pS183L的多克隆抗体。通过ELISA鉴定多克隆抗体效价大于1∶128000;Western blot和间接免疫荧光试验(IFA)表明,该多克隆抗体能识别293T和PK15细胞外源表达蛋白及ASFV感染猪肺泡巨噬细胞(PAMs)内源表达蛋白。综上,本研究成功制备抗pS183L多克隆抗体,为深入研究ASFV pS183L的功能提供了关键生物材料。

摘要：世界范围的珊瑚礁石逐渐地正在变得并且有害地影响在之上由包括的许多因素，重要气候变化，例如全球温暖和增加的 El Nino 南部的摆动活动。通常，珊瑚礁石的坚持，在特别低纬度，部分地，海运被管理不超过集体死亡能在发生的批评限制(∼30 ° C ) 的表面温度。因此，它被想珊瑚那生活在高纬度(即，当前更凉爽的海表面温度) 将多半比珊瑚生活在对假设未来温度增加更有利作出回应低纬度(即，当前更温暖的海表面温度) 。因而，高纬度的珊瑚社区可以有潜力在面临潜在的未来时为许多珊瑚种类充当 refugia 的区域全球温暖。Daya 海湾(22 ° 3 1 ′22 ° 5 0 ′N ) ，北华南海，包含几个高纬度的 non-reefal 珊瑚社区并且在这个区域以内代表 scleractinian 珊瑚的最在北方的分布之一。显著地， Daya 海湾经历了在整个过去的 50 年的在空和海表面温度中的戏剧的温暖。在这篇论文，我们在 Daya 海湾珊瑚社区分析 25 年变化，把两个基于历史性的调查并且我们的最近 2006 2008 地区性的生态的调查。我们的结果建议与预言相反，在过去的 25 年期间在在 Daya 海湾以内的珊瑚盖子有重要衰落(即，在到在 2008 的 15.3% 仅仅珊瑚盖子的 1983/1984 的 76.6% 珊瑚盖子) 。如此的变化也在最丰富的珊瑚种类反映重要移动，从 Acropora pruinosa 到 Favites abdita。大多数现代珊瑚社区变得以前在 15 和 30 年之间建立了，相应于增加的冬季海表面温度的一个时期。然而，很少的殖民地变得在最后 15 年以内建立了，尽管有温暖的一个更强烈的时期。由考虑另外的因素，我们假设那直接人为的影响，而非气候的事件，限制了发展，并且驾驶了衰落，最后 15 年里的 Daya 海湾珊瑚社区。Daya 海湾也在过去的 50 年期间受到了偶尔的极端冷事件，与最近的发生在 2008 年初(13 年 1 月 13 日 2 月) 。在 2008 冷事件期间，最低空气温度到达仅仅 6.6 ° C，和吝啬的海表面温度 2 月秋天，包括在 12.3 ° C 的六连续的天 < 14 ° C。显著地，海表面温度在通常在 scleractinian 珊瑚社区导致集体死亡的假设批评更低的温度阀值(∼13 ° C ) 下面掉落。令人惊讶地，我们的珊瑚社区调查，以前进行了两个(2007 年 8 月) 并且在以后(2008 年 2 月下旬) 极端 2008 冷事件，证明 Daya 海湾珊瑚生态系统恰好被影响在之上在冷时期期间。那些观察建议 Daya 海湾 scleractinian 珊瑚社区开发了改编到低海表面温度。总的来说，我们的数据支持高纬度的珊瑚社区例如 Daya 海湾，有的假设在潜在的未来的来临为 scleractinian 珊瑚充当 refugia 的区域的潜力全球温暖。

摘要：A series of novel triazole derivatives containing y-lactam were designed and synthesized, and their structures were confirmed by IH NMR, 13C NMR and HRMS, The in vitro antifungal activities of the target compounds were evaluated, The results showed that all of the compounds exhibited stronger activity against the six clinically important fungi tested than fluconazole. 3D and 3E showed comparative activity against the fungi tested except for Candida glabrata and Aspergillus fumigatus as voriconazole. In addition, the docking model for 2A and CYP51 was investigated.

摘要：氢能源因具有清洁、高效、可持续等优点使其在能源、交通等领域发展迅速,但是普通民众对于氢气的了解非常有限,依然存在着“谈氢色变”的认识误区。实验以AgNO_(3)和Co(NO_(3))_(2)·6H_(2)O为原料,通过煅烧法制备了Ag-Co_(3)O_(4)金属纳米材料,并以该金属纳米材料为催化剂催化氨硼烷(缩写为“AB”)水解反应制取氢气,之后利用氢燃料电池,将氢气转化为电能实现小车行驶或使组装小电风扇运行。该实验不仅可用于科普展示,也可用于实验教学,应用前景广阔。

摘要：为了消除设计决策者决策偏好对产品开发的影响,进一步提升设计决策效率,提出了ResNet人工智能设计决策模型。该模型基于人工智能思想,构建了基于产品造型语义的设计历史方案数据集,并对该数据集进行了产品造型语义标注。通过深度残差学习网络算法(ResNet)对数据集进行不断训练来提高设计决策的准确度,将一般设计决策问题转化为设计方案图像的语义识别问题,最大限度地消除了决策者决策偏好的影响。通过起重机造型设计决策实例,验证了ResNet人工智能设计决策算法的有效性和可行性。

摘要：The development of efficient photocatalytic H2-evolution materials requires both rapid electron transfer and an effective interfacial catalysis reaction for H2 production. In addition to the well-known noble metals, low-cost and earth-abundant non-noble metals can also act as electron- transfer mediators to modify photocatalysts. However, as almost all non-noble metals lack the interfacial catalytic active sites required for the H2-evolution reaction, the enhancement of the photocatalytic performance is limited. Therefore, the development of new interfacial active sites on metal-modified photocatalysts is of considerable importance. In this study, to enhance the photocatalytic evolution of H2 by Ni-modified TiO2, the formation of NiSx as interfacial active sites was promoted on the surface of Ni nanoparticles. Specifically, the co-modified TiO2/Ni-NiSx photocatalysts were prepared via a two-step process involving the photoinduced deposition of Ni on the TiO2 surface and the subsequent formation of NiSx on the Ni surface by a hydrothermal reaction method. It was found that the TiO2/Ni-NiSx photocatalysts exhibited enhanced photocatalytic H2-evolution activity. In particular, TiO2/Ni-NiSx(30%) showed the highest photocatalytic rate (223.74 μmol h.1), which was greater than those of TiO2, TiO2/Ni, and TiO2/NiSx by factors of 22.2, 8.0, and 2.2, respectively. The improved H2-evolution performance of TiO2/Ni-NiSx could be attributed to the excellent synergistic effect of Ni and NiSx, where Ni nanoparticles function as effective mediators to transfer electrons from the TiO2 surface and NiSx serves as interfacial active sites to capture H+ ions from solution and promote the interfacial H2-evolution reaction. The synergistic effect of the non-noble metal cocatalyst and the interfacial active sites may provide new insights for the design of highly efficient photocatalytic materials.