摘要：The ability to design and synthesize the genome of a living organism marks one of the most significant achievements in synthetic biology. The first synthetic genomes were those of bacteria （Gibson et al., 2010; Hutchison et al., 2016）, but an international consortium of scientists is now undertaking the ambitious task of designing and synthesizing the entire genome of Saccharornayces cerevisiae, a eukaryotic single-celled yeast （Dymond et al., 2011; Kannan and Gibson, 2017）. One feature of the redesigned yeast genome, called Saccharomyces cerevisiae 2.0 or Sc2.0, is the ability to in-duce novel genetic variation.