摘要：Previous studies have suggested that the transforming growth factor- $$\upbeta $$ receptor ALK5 is crucial for articular chondrogenesis by bone marrow mesenchymal stem cells. Here, the wild-type ALK5 plasmids were mutated by overlapping extended PCR and transfected into bone marrow mesenchymal stem cells. The knee joint osteoarthritis mouse model was constructed by cutting off the anterior cruciate ligament and divided into three groups: saline group, bone marrow mesenchymal stem cells and ALK5-transfected bone marrow mesenchymal stem cells group. HE staining showed that the articular cartilage lesions were more serious of saline group compared with that of mesenchymal stem cell group, and this trend was more pronounced as time goes on. Immunohistochemical staining showed that although the expression level of type II collagen in all three groups down-regulated gradually upon time, its expression in ALK5-transfected bone marrow mesenchymal stem cells group was significantly enhanced compared with the other two groups. Micro-CT also suggested that ALK5 transfection of mouse bone marrow mesenchymal stem cells would promote repairing the knee cartilage lesions with arthritis of the mice. Although the osteoarthritis mechanism underlying a variety of factors work together, and the appropriate proportion of ALK5/ALK1 was also emphasized for the treatment of osteoarthritis. This work therefore demonstrated that ALK5 transfection of bone marrow mesenchymal stem cells could be a promising stem cell therapy for repair of cartilage lesions.

摘要：目的:观察优韧瓷高嵌体修复无髓后牙的效果。方法:选取2019年1月至2019年12月就诊的61例患者(67颗无髓后牙)进行前瞻性研究,采用数字化技术设计并制作优韧瓷高嵌体,治疗后1年采用美国公共卫生署修正评估系统评价远期修复效果(边缘密合度、边缘染色、颜色变化、表面质地、修复体完整性、固位、邻接关系及牙体完整性等8项),并比较患牙与对侧同名健康牙的牙龈指数(GI)。结果:修复后1年复查,8项指标成功率均在90.00%以上,高嵌体在边缘密合度、修复体完整性、修复体固位及牙体完整性等方面表现良好,成功率均达100.00%;所有患牙均未发生修复体崩折、脱落或基牙牙体折裂,有5颗发生边缘染色,6颗颜色轻度改变,5颗表面轻度粗糙,4颗邻接过松、有食物嵌塞,边缘染色、颜色变化、表面质地及邻接关系的成功率依次为92.54%、91.04%、92.54%及94.03%;患牙与对侧同名健康牙GI比较,差异无统计学意义(P>0.05)。结论:优韧瓷高嵌体修复无髓后牙在边缘密合度、修复体完整性、修复体固位及牙体完整性方面表现良好,且对牙龈健康的影响较小。

摘要：目的:基于任务态功能磁共振成像技术,通过观察轻度认知障碍(MCI)患者太溪穴针刺态脑神经元活动的改变,探讨针刺对认知相关脑区的神经调节机制。方法:选择轻度认知障碍患者16例,采用16 min任务态实验设计。使用3.0T磁共振扫描仪,首先采集8 min基线静息态数据,再采集8 min针刺太溪穴的针刺态数据。数据经后处理与一般线性模型分析,基于体素水平P=0.001(未校正)和cluster水平P<0.05(FWE校正),获得MCI患者针刺态脑内神经元活动改变的脑功能区。结果:针刺MCI患者右侧太溪穴正激活脑区为左侧眶部额下回(BA47)、右侧三角部额下回(BA45)、左侧岛盖部额下回(BA44)、右侧岛叶(BA13)、左侧颞上回(BA38)、右侧颞下回(BA20)、右侧小脑前叶、后叶、右侧丘脑。负激活脑区为左侧后扣带回(BA31)、左侧楔前叶(BA7)、右侧海马回。结论:针刺MCI患者太溪穴的即刻效应可直接影响认知活动相关脑区的神经元活动,调节病理性失衡的认知回路,可能是针灸对MCI治疗的潜在神经机制。

摘要：为有效防止医用高压氧舱氧气浓度异常对人体造成的损害,设计基于ZigBee的医用高压氧舱氧气浓度自动控制系统。数据采集模块利用微处理器STM32F103C8T6接收各个采集点的氧浓度数据,经数据处理模块的ZigBee将处理结果传输给从机接收端的MSP430G2553单片机,MSP430G2553单片机采用自组织T-S模糊神经网络(SOTSFNN),加入激活强度概念,实现高压氧舱氧气浓度自动控制,并在氧浓度低于19 mg/L、高于23 mg/L时控制蜂鸣器发出报警,通过LCD12864显示当前实时氧浓度。实验结果表明:随着通信距离增加,该系统的丢包率始终低于5%,且相同通信距离下的信号强度较好;该系统能有效控制氧气浓度值在设定范围内,且氧气浓度控制精度高、稳定性好。

摘要：传统的医用高压氧舱供氧故障检修系统在进行供氧故障检测时,检修准确率较低。为此,论文设计基于改进技术(PCA,principal components analysis)的医用高压氧舱供氧故障自动检修系统。通过感知层采集医用高压仓供氧数据信息,利用改进PCA模型实现医用高压氧舱供氧故障检修,依据非线性小波变换阈值法去除数据集噪声,有效保证故障自动检修的准确率。实验结果表明,变量数量为25个时,该系统的降维耗时仅为3.2s;噪声在90dB时,该系统的误检率和漏检率仅为0.92%和0.98%;该系统可提高故障检修准确率,并且抗噪性能良好,可在噪声环境中准确完成故障自动检修。