摘要：The response surface methodology(RSM) combined with bioassays was employed to optimize the extraction process of crude fucose-containing sulphated polysaccharides(c FCSP) from Sargassum fusiforme. The central composite design(CCD) was used with four variables, five levels, and four responses. The four variables were p H value of hydrochloric acid solution, extraction temperature(℃), ratio of liquid to raw material(m L g^(-1)), and extraction time(h), respectively. Chemical and bioassay indices were used in combination as the response parameters, which included the yield of c FCSP, fucose content, proliferation rate of spleen cells, and lipopolysaccharide-induced proliferation of splenocytes. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis, and examined using the appropriate statistical methods. The best extraction conditions were as follows: the p H value of hydrochloric acid solution was 3.50; the extraction temperature was 100℃; the ratio of liquid to raw material was 15.00 m L g^(-1) and the extraction time was 2.50 h. The experimental yield was close to the predicted from the model. The extract could promote spleen lymphocyte proliferation, especially the lipopolysaccharide-induced lymphocyte proliferation in vitro, which suggested that its immunomodulatory effect on B lymphocytes. Therefore, c FCSP extracted from S. fusiforme could be utilized as an immunostimulant in functional foods and pharmaceutical industry in future.

摘要：采取一种有效途径合成了二、三分支的甘露糖簇分子 .甘露糖经烯丙苷化、乙酰基保护后 ,将其烯丙基的双键氧化得到带有羧基连接臂的甘露糖衍生物 ,然后再分别与 1,6 己二胺和三 ( 2 氨乙基 )胺进行缩合反应 ,最后脱掉保护基 ,得到二分枝甘露糖簇化合物 6和三分枝甘露糖簇化合物 8.

摘要：海洋是一个巨大的药源宝库。本世纪 6 0年代以来 ,从海洋动物、植物及微生物中已分离获得新型化合物 10 0 0 0多种 ,其中 1/2以上具有抗肿瘤、抗菌、抗病毒、抗凝血等药理活性 ;这些新型化合物为药物设计提供了可贵的分子模型 ,为海洋药物的开发提供了重要的先导化合物库。然而将这些活性化合物开发成药、用于临床的并不多。究其原因 ,药源是制约其海洋药物产业化的关键因素。纵观海洋药物研究现状、发展趋势 ,基于现有的工作基础 ,我们认为通过半合成手段、结合组合化学原理、利用海洋生物技术进行海洋药物开发是解决药源的关键。

摘要：5-氟尿嘧啶 (5- Fu)以壳聚糖为载体 ,采用乳化化学交联技术 ,制备了 5- Fu壳聚糖微囊。用正交设计安排试验 ,以微囊载药量、药物包封率、粒子分布百分数为指标优化微囊的制备工艺 ,并以最佳制备工艺条件制得含药微囊 ,方法重现性好 ,制备工艺稳定。体外释放度试验表明 ,该微囊具有较好的缓释作用。

摘要：胃肠双重造影的特殊价值，在于能观察胃肠组织的微细解剖结构，并可发现早期病变，如早期胃癌及微小溃疡等。但所用钡剂质量要求较高，其具体指标要求：