摘要：针对当前CCUS-EOR数字化系统中注入、驱油、埋存、采出、集输、回注各环节的数据和功能模块分散独立、协同性差等问题,设计了CCUS-EOR油藏井筒地面一体化生产协同管控系统。按照工业互联网顶层设计要求,采用微服务和容器化技术,构建了CCUS-EOR管控系统的架构和标准化的数据模型,设计了数据与模型管理、方案设计、生产运行三个功能单元,包括生产监控、油藏动态、注采井筒、地面集输、协同调控、数据管理等应用模块。研究表明:构建的CCUS-EOR生产协同管控系统可实现CCUS-EOR全流程数据融合、生产监控、工况诊断、完整性预警、协同决策、智能调控,为CCUS-EOR安全生产和提高采收率提供数据和平台支撑。

摘要：Objective： In our previous work, we prepared a type of chitosan hydrogel with excellent biocompatibility. In this study, tissue-engineered cartilage constructed with this chitosan hydrogel and costal chondrocytes was used to repair the articular cartilage defects. Methods： Chitosan hydrogels were prepared with a crosslinker formed by combining 1,6-diisocyanatohexane and polyethylene glycol. Chitosan hydrogel scaffold was seeded with rabbit chondrocytes that had been cultured for one week in vitro to form the preliminary tissue-engineered cartilage. This preliminary tissue-engineered cartilage was then transplanted into the defective rabbit articular cartilage. There were three treatment groups： the experimental group received preliminary tissue-engineered cartilage; the blank group received pure chitosan hydrogels; and, the control group had received no implantation. The knee joints were harvested at predetermined time. The repaired cartilage was analyzed through gross morphology, histologically and immunohistochemically. The repairs were scored according to the international cartilage repair society （ICRS） standard. Results： The gross morphology results suggested that the defects were repaired completely in the experimental group after twelve weeks. The regenerated tissue connected closely with subchondral bone and the boundary with normal tissue was fuzzy. The cartilage lacuna in the regenerated tissue was similar to normal cartilage lacuna. The results of ICRS gross and histological grading showed that there were significant differences among the three groups （P〈0.05）. Conclusions： Chondrocytes implanted in the scaffold can adhere, proliferate, and secrete extracellular matrix. The novel tissue-engineered cartilage constructed in our research can completely repair the structure of damaged articular cartilage.

摘要：背景与目的非小细胞肺癌(non-small cell lung cancer, NSCLC)作为肺癌的一种,由于其高发病率一直备受关注。准确揭示其发病机制对于NSCLC的诊断以及治疗具有重要的指导意义。MATF-A作为转录调控因子在多种肿瘤的发生发展过程中发挥着重要作用,可以调控多种肿瘤细胞的迁移过程。HOTAIR是近年研究发现的一个长链非编码RNA,在多种肿瘤中异常表达并参与多种肿瘤的增殖及迁移的进程。本研究旨在探究MRTF-A通过HOTAIR调控NSCLC的增殖及迁移进程。方法构建MRTF-A的过表达质粒及干扰质粒,检测对A549细胞增殖及迁移的影响。设计合成HOTAIR的si RNA检测对A549细胞增殖及迁移的作用。q RT-PCR检测MRTF-A对HOTAIR表达的调控作用。构建HOTAIR的启动子检测MRTF-A对HOTAIR启动子活性的影响。结果过表达MRTF-A促进A549细胞的增殖,沉默MRTF-A的表达抑制A549细胞的增殖及迁移。干扰HOTAIR的表达抑制A549细胞的增殖及迁移。MRTF-A能够影响HOTAIR的表达,同时能够调控HOTAIR启动子的活性。结论 MRTF-A通过HOTAIR调控NSCLCA549细胞的增殖及迁移进程。

摘要：从隧道管理者角度出发,以长大公路隧道为研究对象,筛选出能够反映长大公路隧道运营管理情况的评价指标,建立一套科学的、适合长大公路隧道的安全评价体系。在研究的理论成果基础上开发相应的长大公路隧道运营综合评价软件系统。

摘要：In this paper, we present a novel design scheme of temperature-jump(T-jump) area for microfluidic *** simulation and experimental research of thermal characteristics of the solution in microchannels is *** simulation of the temperature-jump microchannel is analyzed to study the heat transfer characteristics by comparing performance of three proposed *** of the power requirement is discussed in the dimensional design of microheater. Temperature-sensitive fluorescent dye is applied to investigate the temperature field of microchannel heated by a designed microheater. It is found that the T-jump microfluidic device can provide rapid heating for solutions with strong convection heat transfer ability.

摘要：随着中国城市化进程不断加快,土地资源的利用愈加紧张,大量的地下空间得到了充分利用。如今,在中国各大城市,高层建筑、商业综合体、地下车库等建筑群体已成为城市发展的重要组成部分。而在这些建筑的建设过程中,往往需要开挖深基坑,以满足建筑的需要。深基坑的建设不仅能够提高土地的利用效率,还可以降低建筑成本并增加项目的投资回报率。然而,在深基坑的施工中,由于工程规模大、难度较大,其施工风险也相对较高,因此深基坑支护技术的研究和实践具有重要意义。为了保证深基坑建设的安全性和可靠性,需要对深基坑支护施工技术进行充分研究。论文从深基坑支护施工技术的概念、施工过程进行了论述。同时,分析了影响深基坑支护施工质量的因素,以期为深基坑支护施工提供一些参考和借鉴意义。

摘要：在万物互联的大背景下,实现系统的安全可信始终是最核心的目标。随着国产信息系统建设的不断深入,实现系统的安全可信已经成为最迫切的需求。为了解决工业互联网的多接口数据互联和数据安全问题,设计了一款基于飞腾嵌入式E2000处理器的安全网关设备。实现了3G/4G/5G无线VPN路由功能,同时支持以太网、RS232/485串口,CAN口接入,并支持Wifi/蓝牙通信以及GPS/北斗定位。通过分析VLAN协议原理,基于RTL8367SC芯片实现了内置网络交换机功能;融合基于RG200U的5G模块挂载方法,形成了网关的内置5G交换机系统,进一步解决了多通道数据互联的问题。通过分析飞腾安全处理器平台架构规范(Phytium Security Platform Architecture,PSPA),设计了网关的安全可信固件制作方法,保证了启动过程执行的所有代码都是安全可信的。通过搭建测试环境进行测试验证,测试结果表明,该系统实现了多种接口的数据互联,并基于多项E2000芯片内置安全加密算法,使安全性方面得到了较大提升。

摘要：We study the distributed Kalman filtering problem in relative sensing networks with rigorous *** relative sensing network is modeled by an undirected graph while nodes in this network are running homogeneous dynamical models. The sufficient and necessary condition for the observability of the whole system is given with detailed proof. By local information and measurement communication, we design a novel distributed suboptimal estimator based on the Kalman filtering technique for comparison with a centralized optimal estimator. We present sufficient conditions for its convergence with respect to the topology of the network and the numerical solutions of n linear matrix inequality（LMI） equations combining system parameters. Finally, we perform several numerical simulations to verify the effectiveness of the given algorithms.

摘要：背景:血管内皮生长因子受体对许多实体瘤的血管生成和预后起着重要作用,但血管内皮生长因子受体与肺癌预后的关系研究报道较少.目的:探讨肺癌中血管内皮生长因子受体Flt1和KDR的表达及其与转移及预后的关系.设计:以病理标本为研究对象单一样本研究.单位:一所大学的病理学教研室.材料:随机选取青岛大学医学院附属医院胸外科1985-01/1990-12经手术切除的75个肺癌标本.2002-09/11月间在青岛大学医学院病理学教研室进行实验.方法:应用免疫组织化学Power Vision TM-9000(PV-9 000)法,测定75个肺癌标本中Flt1和KDR的表达.主要观察指标:肺癌中血管内皮生长因子受体Flt1和KDR的表达及其与年龄、性别、病理类型、组织学分级、肿瘤大小、淋巴结转移和预后的关系.肿瘤细胞、纤维母细胞中Flt1和KDR表达的关系.结果:肺癌组织中Flt1和KDR的表达较为广泛,主要位于肿瘤细胞胞浆及胞膜上,纤维母细胞和血管内皮细胞胞浆中亦有表达.Flt1和KDR在肿瘤细胞中的阳性率均显著高于在间质纤维母细胞中的表达(x2=6.07,5 88,P＜0.05).肿瘤细胞及纤维母细胞中该两种受体的阳性率在不同年龄、不同性别及不同病理类型、不同病理分级之间差异均无显著性意义(P＞0 05).肿瘤细胞中Flt1和KDR的阳性表达率在3组不同大小的肿瘤间差异均有显著性意义(x2=10 35,7.29,P＜0.05),而纤维母细胞中差异均无显著性意义(x2=2 86,2.56,P＞0 05);肿瘤细胞及纤维母细胞中Flt1和KDR的阳性率在淋巴结有、无转移2组间的差异均有显著性意义(x2=4.72～9.32,P＜0.05),在3组不同术后生存时间患者间差异亦均有显著性意义(x2=8 81～19.19,P＜0.05);肿瘤细胞中Flt1和KDR的表达呈极显著性正相关(r=0.44,P＜0.01).结论:Flt1和KDR在肿瘤细胞中的表达均显著高于间质纤维母细胞,提示肺癌的生长主要依赖自分泌机制;肿瘤细胞中该两种受体的表达与淋巴结转移、术后生存时间有关,并具有协同效应,提示联合检测Flt1和KDR的表达,可能对肺癌转移及预后的评估更有参考价值.

摘要：针对新能源特性与油气连续生产制度不匹配、常规装备及工艺与油田智能生产不适应、当前生产管控制度与协同生产管控及能源综合利用不完备的问题,开展了低碳零碳智能采油气井场新型建设模式探讨。文章分析了采油气井场生产用能特征,设计了新型的绿色智能采油气井场生产用能供配能模式。研究了油气生产多能微网协同管控关键技术,即实时感知、预测新能源供电功率和生产用能侧需求,协同优化源—网—荷—储配置及运行调度,通过微网优化控制器进行多能源耦合互补、多元负荷协同调控,提升油气生产能源使用的计划性和效率,减少弃风、弃光、弃热,解决了清洁替代中能源转换高成本、现有采油气工艺不适应新能源特点、消纳能力低的难题。实例分析了低碳零碳智能油气井场实践案例,指出了“光风能+直流母线+智能间开+电机柔性驱动”组合模式是解决低产低效抽油机井群生产提质增效的技术创新,助力了采油气生产实现从“安全高效”到“低碳智能”的跨越。