摘要：The aim of this study was to optimize the conditions for the extraction of low-abundance proteins（LAPs） and the removal of abundant proteins（APs; β-conglycinin and glycinin） from soybean *** factor and orthogonal experiments were designed to determine the effects of four factors（isopropanol concentration, total extraction time, ultrasonic power, and ultrasonic time） on protein concentration in isopropanol *** in the isopropanol supernatant and the cold acetone precipitate of isopropanol were identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE） and matrix-assisted laser desorption/ionization-time of flight mass spectrometry（MALDI-TOF-MS）.The results showed that the optimal conditions were 50% isopropanol, ultrasonic pretreatment for 15 min at 350 W, and a total extraction time of 1 *** these conditions, the protein concentration in the isopropanol extracts reached 0.8081 g/*** LAPs were detected, including β-amylase, soybean agglutinin, soybean trypsin inhibitor, fumarylacetoacetase-like, phospholipase D alpha 1-like, oleosin, and even some unknown soybean *** soybean APs（β-conglycinin and glycinin） were not *** method may be useful for discovering new soybean proteins and extracting enough LAPs of soybean to allow further studies of their physiological effects on animals without the influence of APs.

摘要：重庆石板坡长江大桥复线桥主跨(330 m)的103 m钢箱梁段为整体吊装合龙,在温度变化下的大尺寸合龙是技术难度较高的工作。介绍在桥梁设计中对合龙的考虑和实施结果。

摘要：合成了6-((2H-四唑-5-基)-氨基)-1,2,4,5-四嗪-3(2H)-酮(TATzO)并通过红外光谱、元素分析、核磁和单晶X射线衍射对其结构进行了表征。单晶结构表明,TATzO·H2O属于正交晶系,空间群是Pnma,密度为1.730 g·cm-3。在非等温条件下,利用差示扫描量热法(DSC)和热重(TG-DTG)研究了TATzO的热分解行为并计算得到分解峰温、活化能(E)、指前因子(A)、热点火温度(Tbe)和热爆炸临界温度(Tbp),分别为230.46℃、169.03 kJ·mol^-1、15.65 s^-1、213.75℃和223.03℃。TATzO·H2O分解峰温和热爆炸临界温度与传统含能材料RDX相近,表明TATzO热稳定性较高。通过高斯03软件包设计等键反应计算生成焓(HOF),由Kamlet-Jacobs(K-J)方程计算爆速(D)和爆压(p)以评估爆轰性能。D和p分别为7757 m·s^-1和25.74 GPa。落锤法测得TATzO撞击感度大于24 J。

摘要：以实现故障诊断的应用实践为出发点 ,提出以信息的模型描述为基础 ,采用软件工程学技术和数据仓库原理 ,以兼容性、通用性与可再编程为标准的故障诊断信息系统设计原则 ,首次实现了接收和运用列车车载微机检测控制系统所记录的运行状态信息 。

摘要：Dysregulated HGF/c-Met signalling has been associated with many human cancers,poor clinical outcomes,and even resistance acquisition to some approved targeted *** such,c-Met kinase has emerged as an attractive target for anticancer drug discovery.

摘要：Li-metal anodes are one of the most promising energy storage systems that can considerably exceed the current technology to meet the ever-increasing demand of power applications. The apparent cycling performances and dendrite challenges of Li-metal anodes are highly influenced by the interface layer on the Li-metal anode because the intrinsic high reactivity of metallic Li results in an inevitable solid-state interface layer between the Li-metal and electrolytes. In this review, we summarize the recent progress on the interfacial chemistry regarding the interactions between electrolytes and ion migration through dynamic interfaces. The critical factors that affect the interface formation for constructing a stable interface with a low resistance are reviewed. Moreover, we review emerging strategies for rationally designing multiple-structured solid-state electrolytes and their interfaces, including the interfacial properties within hybrid electrolytes and the solid electrolyte/electrode interface. Finally, we present scientific issues and perspectives associated with Li-metal anode interfaces toward a practical Li-metal battery.

摘要：随着农业机械的技术进步与创新,传统材料已不能满足农业机械提升技术性能和经济性能的要求,正确选择和应用新型材料,成为现代农业机械研究设计中的重要技术课题。通过分析镁合金的特性和优势,探讨了在农业机械装备领域应用的必要性、重点方向和未来前景。

摘要：The partial 16 S r RNA gene sequences（100 to 500 bp） were widely used to reveal rumen bacterial composition influenced by diets, while quantification of the changed uncultured bacteria was inconvenient due to difficult designing of specific primers based on short sequences. This study evaluated the effect of forage resources on rumen bacterial diversity and developed new strategy for primer design based on short sequences to quantify the changed uncultured bacteria. Denaturing gradient gel electrophoresis（DGGE） analysis and subsequent band sequencing were used to reveal the distinct rumen bacteria composition in cows fed with two forage sources（single corn stover vs. mixed forages including alfalfa hay and corn silage）. The bacterial diversity in the rumen of dairy cows fed with corn stover was lower than that with mixed forages（P0.05）. The bacterium named R-UB affiliating to uncultured Succinivibrionaceae was identified, and it was abundant in the rumen of cows fed with mixed forages compared to corn stover. The full length 16 S r RNA gene sequences with identity of 97% to the R-UB 16 S r RNA gene sequence were obtained from Gen Bank and used to design specific primers to quantify uncultured bacterium R-UB. All sequences of amplicon from the new primers were of 100% identity to R-UB sequences indicating the high specificity of new primers. Quantitative PCR confirmed that abundance of R-UB in the rumen of cows fed with corn stover was lower than those fed with mixed forages（P0.01）. New strategy for designing primers based on partial 16 S r RNA genes to quantify targeted uncultured bacteria was successfully developed. The rumen bacteria descending significantly in the cows fed corn stover compared to those fed mixed forages was identified as uncultured R-UB from Succinivibrionaceae.

摘要：为应用NARX神经网络识别路面不平度,对NARX神经网络及其训练过程和结构设计进行了分析,采用相关系数和均方根误差作为NARX神经网络识别效果的评价指标。建立了路面不平度滤波白噪声模型和汽车平顺性4自由度平面模型,通过仿真获得路面不平度和车辆响应。以可测试的车辆响应作为NARX神经网络的输入,采用正交试验设计提出确定NARX神经网络输入方案的方法,对在常用等级路面上以常用车速行驶的某汽车的前轮路面不平度进行了识别。结果表明,将可测试的车辆响应作为NARX神经网络输入,结合正交试验设计,解决了NARX神经网络最优输入方案的确定问题。

摘要：为了提高打击大机动目标命中率、机动性和燃油利用率,设计一种适用于制导控制一体化的模糊滑模方法.建立拦截导弹制导控制一体化模型,选取零控脱靶量作为滑模面,将制导律嵌入控制器的设计中,并将运动学关系与动力学特性有机融合.在滑模控制器中加入模糊环节,有效克服了滑模方法的抖振问题.目标蛇形机动的弹道仿真结果表明,所提出方法可以有效提高系统各方面的性能.