摘要：Data collection with microcrystals at synchrotron radiation facilities is challenging because it is difficult to harvest and locate microcrystals. Moreover,microcrystals are sensitive to radiation damage; thus, typically, a complete data set cannot be obtained with a single microcrystal. herein, we report a new method for data collection with multiple microcrystals having a crystal size ranging from 1 to 30 lm. This method is suitable for not only low-temperature(100 K) data collection but also room-temperature data collection. Thin Kapton membranes were used to capture multiple crystals simultaneously. The microcrystals were visible under an optical microscope and easily located because the membrane was transparent and sufficiently thin. The film was fixed to a bracket that was prepared using a three-dimensional printer. The bracket was fixed on a magnetic base via screwing and employed by the goniometer system for data collection. Multiple data sets of fatty acid-binding protein 4(FABP4) and lysozyme microcrystals were collected using this novel designed device. Finally, the structures of protein FABP4 and lysozyme were obtained from these data via the molecule replacement method. The data statistics reveal that this method provides a comparable result to traditional methods such as a nylon loop.

摘要：目的探讨儿童单纯脊髓栓系综合征(TSC)小切口松解术的安全性及有效性。方法选取2017年12月—2020年12月宁波大学医学院附属医院收治的82例单纯TSC患儿,将其随机分为对照组和观察组,各41例。所有患儿仅采用终丝切断术,其中对照组患儿采用传统手术,观察组患儿采用小切口松解术,并术后随访6个月。比较两组患儿住院时间、切口大小;术后及随访终点的临床疗效;手术前后及随访6个月的视觉模拟评分(VAS)、日本骨科协会评估量表(JOA)评分变化;术后并发症。结果观察组患儿住院时间短于对照组(P0.05)。观察组与对照组患儿术前、术后、随访6个月的VAS、JOA评分比较,采用重复测量设计的方差分析,结果:①不同时间点的VAS、JOA评分有差异(F=2.507和4.142,P=0.014和0.000);②观察组与对照组VAS、JOA评分有差异(F=4.913和2.229,P=0.000和0.029);③两组VAS、JOA评分变化趋势有差异(F=35.944和167.373,均P=0.000)。观察组患儿术后并发症总发生率低于对照组(P<0.05)。结论小切口松解术治疗儿童单纯TSC疗效显著,可缩短住院时间,有效缓解疼痛,改善腰椎功能,且安全性良好。

摘要：针对圆形地下连续墙基坑开挖受力过程复杂且采用有限元通用程序计算效率低的问题,分析了墙体承受的内衬环向支撑力、坑外水平土压力荷载、坑内水平地基反力计算方法,基于轴对称荷载作用下柱壳的三维弹性力学基本方程,建立了圆形地下连续墙墙体的形变势能、内衬弹性势能、水平土压力势能、水平地基反力弹性势能等的计算公式,从而得到了求解墙体位移问题的泛函;引入单傅里叶级数作为求解位移的试探函数,根据变分原理对试探函数的待定参数进行全微分,得到了待定参数的求解方程组;同时,给出了各种边界条件下的待定参数方程组,从而得到了圆形地下连续墙的里兹解法;最后采用里兹法和有限元法对外径73m的黄埔大桥北锚碇圆形地下连续墙进行了分析对比。结果表明:里兹法与三维弹性地基板法有限元计算的施工阶段最大位移仅相差0.49%,最大弯矩仅相差1.2%,计算结果几乎一致,尤其是位移曲线几乎完全重合,说明了里兹法方法的正确性,且其有很高的计算精度;算例中里兹法计算的未知数为17个,而有限元计算节点数为40 075个,未知数为240 450个,可见里兹法计算效率非常高,非常适合编制小型程序求解;研究结果为圆形地下连续墙受力研究提供了一种新方法,可广泛应用于工程设计。

摘要：数据库服务器对于客户端请求都有一个最大的连接数,这限制了数据库事务处理性能,尤其在对一些具有终端操作的事务处理类型的应用,每分钟能处理的事务数不高,表现为TPC-C测试中的tpmC的性能值偏低。本文提出了提高数据库TPC-C测试的tpmC值的两种中间件的策略,在传统的连接池技术的基础上,提出了降低调度粒度,以事务乃至语句——而不是客户端请术——为调度单位的中间件模型,从而可提高数据库的事务处理效率。

摘要：Fishes interact with the fluid environment using various *** multiple control surfaces work in combination to produce the thrust and the balance torques in a steady swimming,to maneuver and to position themselves accurately even in turbulent *** motivate us to embark on a research program designed to develop an agile biologically inspired robotic fish based on the performance of multiple *** accomplish this goal,a mechanical ray-like fin actuated by the shape memory alloy (SMA) is developed,which can realize the oscillating motion,the undulating motion or even the complex three dimensional *** basic unit is the two opposite side SMA-driven plate,namely the fin *** a result,a lightweight bio-inspired fin is constructed by placing radially multiple SMA fin rays.A biologically inspired underwater vehicle (BIUV) is later built using the above multiple lightweight bio-inspired *** common arrangement styles of multiple fins on the BIUV are considered here:one is the posterior fin (for the oscillating motion) that is parallel to the anterior fins (for the undulating motion),another one is the posterior fin that perpendicular to the anterior *** kinematic modeling,the deformation modeling and the detecting of the SMA fin are *** thrust generation is also ***,an experiment is conducted to test the performance of the proposed two arrangement styles,including the comparison of the averaged propulsion velocity and the averaged thrust under certain kinematic ***,the influence of the frequency and the amplitude of the SMA fin ray on the propulsion performance is also investigated.

摘要：Flow rate and temperature are important parameters for design and operation of liquid lead–bismuth(Pb Bi) experimental loop. The PREKY facility was designed to study test technique of flow rate and local temperature of Pb Bi loop. In this work, flow rate monitoring of molten Pb Bi was performed, and temperatures of the Pb Bi and pipe surface were measured. The results show that the flow rate of the venturi-nozzle flow meter had an uncertainty of ±5 % in the range of 0.6–2.0 m/s, and the maximum temperature difference between the Pb Bi and pipe surface was about 8 ℃.

摘要：甘蔗组织培养技术是加速繁殖新品种和加快新品种大面积推广的主要手段,不同品种的离体培养中其培养基中激素配比存在差异,因此筛选合适培养基是提高品种快繁效率的关键。本文以桂糖48号为材料,以MS基本培养基加外源激素浓度梯度进行试验处理设计,对品种的愈伤组织诱导、愈伤分化、试管苗增殖和生根阶段进行培养基筛选试验,以其筛选出最合适桂糖48号的培养基配方。试验结果表明:诱导愈伤组织培养基为MS+2,4-D 3.0 mg/L;愈伤组织分化培养基为MS+BA 1.0 mg/L;初代增殖培养基为MS+BA 2.0 mg/L+NAA 0.1 mg/L+GA 0.5 g/L;继代培养基为MS+BA 2.0 mg/L+NAA 0.1mg/L;生根培养基为1/2 MS+NAA 12 mg/L。在该培养基下,生根最好,苗绿,比较高,长势好,对加快甘蔗新品种桂糖48号在广西大面积推广起到积极的作用。

摘要：The ship design process model is the basis for developing the ship collaborative design system under network *** to the characteristics of the ship design, a method for dividing the ship design process into three layers is pat forward, that is project layer, design task layer and design activity layer, then the formalized definitions of the ship design process model, the decomposing principles of the ship design process and the architecture of the ship collaborative design (SDPM) system are presented. This method simplifies the activity network, makes the optimization and adjustment of the design plan convenient and also makes the design process easier to control and change, at last the architecture of the ship collaborative design system is discussed.

摘要：研究目的:满足运输服务功能,优化运输径路,使车站功能与运输便捷性、铁路枢纽近远期规划发展相适应且节省工程投资,是铁路枢纽大型客运站设计重点考虑的因素。本文通过提出大型车站设计原则及思路,研究南昌铁路枢纽新建大型客运站—南昌西客站的设计思路、站型布置特点,对运营适应性进行分析,检验设计方案与实际运营的符合性。研究结论:(1)提出了新建大型客运站设计原则及思路,指出运营中结合设计方案特点办理有关列车进路,可提高车站作业能力;(2)车站一端咽喉区外立交疏解与车站内平面转场相结合的特殊设计,可实现枢纽大型客运站的运输服务功能;(3)加强与运营一线生产人员交流和沟通,可提升细节设计,提高段、所作业能力;(4)本研究成果可为大型站、段所的规划设计、运营提供借鉴和参考。

摘要：To design a promising Al−Si filler alloy with a relatively low melting-point,good strength and plasticity for the Cu/Al joint,the Cu,Ni,Zr and Er elements were innovatively added to modify the traditional Al−Si eutectic *** microstructure and mechanical properties of filler alloys and Cu/Al joints were *** result indicated that the Al−Si−Ni−Cu filler alloys mainly consisted of Al(s,s),Al_(2)(Cu,Ni)and Si(s,s).The Al−10Si−2Ni−6Cu filler alloy exhibited relatively low solidus(521℃)and liquidus(577℃)temperature,good tensile strength(305.8 MPa)and fracture elongation(8.5%).The corresponding Cu/Al joint brazed using Al−10Si−2Ni−6Cu filler was mainly composed of Al_(8)(Mn,Fe)_(2)Si,Al_(2)(Cu,Ni)3,Al(Cu,Ni),Al_(2)(Cu,Ni)and Al(s,s),yielding a shear strength of(90.3±10.7)*** joint strength was further improved to(94.6±2.5)MPa when the joint was brazed using the Al−10Si−2Ni−6Cu−0.2Er−0.2Zr filler ***,the(Cu,Ni,Zr,Er)-modified Al−Si filler alloy was suitable for obtaining high-quality Cu/Al brazed joints.