摘要：BACKGROUND： The potential application of decellularized liver scaffold for liver regeneration is limited by severe shortage of donor organs. Attempt of using heterograft scaffold is accompanied with high risks of zoonosis and immunological rejection. We proposed that the spleen, which procured more extensively than the liver, could be an ideal source of decellularized scaffold for liver regeneration. METHODS： After harvested from donor rat, the spleen was processed by 12-hour freezing/thawing ×2 cycles, then circulation perfusion of 0.02% trypsin and 3% Triton X-100 sequentially through the splenic artery for 32 hours in total to prepare decellularized scaffold. The structure and component characteristics of the scaffold were determined by hematoxylin and eosin and immumohistochemical staining, scanning electron microscope, DNA detection, porosity measurement, biocompatibility and cytocompatibility test. Recellularization of scaffold by 5×106 bone marrow mesenchymal stem cells（BMSCs） was carried out to preliminarily evaluate the feasibility of liver regeneration by BMSCs reseeding and differentiation in decellularized splenic ***： After decellularization, a translucent scaffold, which retained the gross shape of the spleen, was generated. Histological evaluation and residual DNA quantitation revealed the remaining of extracellular matrix without nucleus and cytoplasm residue. Immunohistochemical study proved the existence of collagens I, IV, fibronectin, laminin and elastin in decellularized splenic scaffold, which showed a similarity with decellularized liver. A scanning electron microscope presented the remaining three-dimensional porous structure of extracellular matrix and small blood vessels. The poros-ity of scaffold, aperture of 45.36±4.87 μm and pore rate of 80.14%±2.99% was suitable for cell engraftment. Subcutaneous implantation of decellularized scaffold presented good histocompatibility, and recellularization of the splenic scaffold demonstr

摘要：“一带一路”国际文化创意园雕塑艺术馆作为呼和浩特市城市新文化地标性建筑,其外墙为保温一体清水混凝土墙体,局部为弧形墙体,极具艺术气息,符合雕塑艺术馆的特有气质。同时对清水混凝土墙体一次性成活的施工工艺及质量控制要求较高,通过对清水混凝土的原材料控制、配合比设计、钢筋、模板、混凝土浇筑等多种途径进行清水混凝土质量控制,提出针对超高、超厚保温一体清水混凝土墙体、弧形清水混凝土墙体质量的施工方法,可为类似工程施工提供参考。

摘要：目的设计并加工用于胸腔镜食道肿瘤术中标记定位的磁体,通过实验犬对磁示踪技术(MTT)的可行性和安全性进行验证。方法用于食道肿瘤标记定位的磁示踪磁体包括示踪磁体(TM)及寻踪磁体(PM)两部分,采用钕铁硼(NdFeB)材料加工而成。选择6只雄性健康Beagle犬,全身麻醉后通过内镜下软组织夹将TM钳夹并固定于食管假定的肿瘤旁边,24 h后行胸腔镜,经操作孔置入PM于食道的可疑病变附近,PM与TM因磁力相互吸引,从而实现食管胸腔镜下手术时对肿瘤的定位。结果成功加工出可用于胸腔镜食道肿瘤术中标记定位的磁TM。6只Beagle犬在内镜下均成功留置TM于食管假定的肿瘤边缘,操作中无磁体滑脱、出血等副损伤。24 h后,胸腔镜手术下顺利置入PM,且PM与TM成功完成自动相吸,实现了胸腔镜下对食道肿瘤的定位和识别,整个过程操作顺利,未出现任何副损伤。结论基于MTT的胃镜联合胸腔镜食道肿瘤标记定位安全可行,具有临床应用价值。

摘要：针对采用液体推进剂的空间推进系统,提出了一种基于图论和面向对象技术的通用数值仿真方法,该方法适用于空间推进系统各种形式设计方案的检验及推进系统静态工作过程的仿真研究。应用此方法开发的软件对某空间推进系统进行了静态特性的仿真,获得良好效果,显示了很好的通用性。

摘要：由于实际工艺条件往往与理想值存在一定偏差,制品在成形后通常会存在一些难以避免的缺陷,而残余应力正是最为常见的一种。注塑制品的残余应力是在注塑成形过程中,外界约束去除后,仍存在于塑件内部的应力,在后期使用过程中容易引起塑件的翘曲、变形、乃至断裂破损,多数情况下对制品不利。因此,在塑料制品设计时应充分考虑到残余应力的影响。本文较为全面地介绍了注塑制品残余应力的产生、检测、减小残余应力的措施及残余应力的计算机模拟等内容。

摘要：面向可重构密码流处理器RCSP,设计并实现基于SystemC语言的全系统模拟器RCSPSim,提出模拟器的硬件资源建模方法、指令集及译码方案和性能统计方案,实现对RCSP处理器的高效精确模拟。RCSPSim能够准确实现密码算法,进行详细的吞吐率评测,为实际处理器开发和优化提供重要的评估和借鉴作用。在单簇(核)前提下,与其它结构的专用密码处理器相比较,RCSPSim在实现典型密码算法时具有较高的吞吐率,验证了可重构密码流体系结构的性能优势。

摘要：In this paper,we deduced an iteration formula for the computation of central composite *** using the iteration formula,the computational complexity of uniform design construction in flexible region can be greatly *** we also made a refinement to threshold accepting algorithm to accelerate the algorithm's convergence *** show that the refined algorithm can converge to the lower discrepancy design more stably.

摘要：目的:构建靶向人类白细胞抗原G1(HLA-G1)的小干扰RNA(siRNA)真核表达载体(pSUPER-HLA-G1),并检测其在滋养细胞系HTR-8/SVneo中的敲减效率。方法:将设计的HLA-G1 siRNA寡聚脱氧核苷酸链与真核表达载体pSUPER连接,构建重组pSUPER-HLA-G1真核表达载体,并用脂质体法将其转染入HTR-8/SVneo细胞系中。pSUPER-HLA-G1质粒转染后采用RT-PCR检测HLA-G1在HTR-8/SVneo细胞中的基因转录情况,Western blotting检测HLA-G1蛋白的表达情况。结果:构建的真核表达载体pSUPER-HLA-G1可在HTR-8/SVneo细胞中表达,HLA-G1 mRNA和其蛋白表达抑制率分别为74.85±7.43%和71.07±6.11%。结论:构建的人HLA-G1 siRNA蛋白真核表达载体pSUPER-HLA-G1有效地沉默了HLA-G1在HTR-8/SVneo滋养细胞中的基因表达及蛋白表达,为今后以HLA-G1为靶点的基因研究奠定了基础。

摘要：为了提高城轨列车再生能量利用率,本文提出一种基于节能的协同控制优化方法,建立以停车精度和准时性为约束条件、以协同时间最长和协同次数最多为优化目标的控制决策模型.根据列车速度、位移方程,求出列车运行工况转换点速度和时刻的解析解;结合积分变量对停车精度和运行时间的影响,确定积分变量的取值范围,将积分变量与列车运行工况转换点关联起来,设计列车运行工况转换点的优化调节策略,并计算目标速度曲线;分析城轨列车牵引工况和制动工况重叠时间的影响因素,得到列车一次牵引最大速度对协同时间的影响规律,设计协同控制策略,求解列车二次牵引时刻及对应一次牵引最大速度.最后,以国内某条地铁线路为背景设计算例,验证了该模型的有效性.

摘要：文章简要叙述了CAE技术在铝型材挤压模具设计优化中的意义 。